Invitrogen

Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eBioscience™

1 Published Figure
2 References
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-9015-82

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

0.1 µg/mL
-

Flow Cytometry (Flow)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

M4G3LN

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572915

Product Specific Information

Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.

Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated M4G3LN is recommended for use in intracellular flow cytometry.).

Applications Tested: This M4G3LN antibody has been tested by immunoblotting of stimulated Daudi B cells. This can be used at less than or equal to 5 µg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: agammaglobulinaemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; homolog of mouse T-cell itk/tsk; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMB; Kinase EMT; Kinase TLK; T-cell-specific kinase; truncated Bruton agammaglobulinemia tyrosine kinase; Tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB); tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14); Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; X-linked immune deficiency

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Gene Aliases: AGMX1; AI528679; AT; ATK; BPK; BTK; EMT; IMD1; ITK; LPFS1; LYK; PSCTK1; PSCTK2; Tcsk; Tlk; Tsk; xid; XLA

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UniProt ID: (Human) Q06187, (Mouse) P35991, (Human) Q08881, (Mouse) Q03526

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Entrez Gene ID: (Human) 695, (Mouse) 12229, (Human) 3702, (Mouse) 16428

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Function(s)
protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding identical protein binding metal ion binding nucleotide binding protein kinase activity lipid binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
negative regulation of cytokine production adaptive immune response regulation of B cell cytokine production MyD88-dependent toll-like receptor signaling pathway regulation of B cell apoptotic process transcription, DNA-templated protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway I-kappaB kinase/NF-kappaB signaling mesoderm development peptidyl-tyrosine phosphorylation calcium-mediated signaling intracellular signal transduction peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway B cell activation regulation of cell proliferation innate immune response positive regulation of B cell differentiation cell maturation B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity apoptotic signaling pathway cytokine production NK T cell differentiation cellular defense response signal transduction activation of phospholipase C activity interferon-gamma production interleukin-4 production T cell activation T cell receptor signaling pathway immune system process histamine secretion by mast cell regulation of transcription, DNA-templated apoptotic process response to organic substance phosphorylation cell differentiation cellular response to reactive oxygen species protein autophosphorylation cellular response to molecule of fungal origin
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