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Phospho-Btk (Tyr223) Antibody (711720) in ICC/IF
For immunofluorescence analysis, RAMOS cells were fixed and permeabilized for detection of endogenous BTK pY223 using Anti-BTK pY223 Recombinant Rabbit Superclonal™ Antibody (Product # 711720, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection a... View More
Product Details
711720
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Peptide corresponding to Human BTK (aa Chemically synthesized phosphopeptide derived from the region of human BTK that contains tyrosine 223 (Antigen similar to 44-1350G))
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2632973
Product Specific Information
This antibody is predicted to react with Monkey, elephant and wild boar.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily of tyrosine kinases. Mutations in the BTK gene have been linked to severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: agammaglobulinaemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; MGC126261; MGC126262; OTTHUMP00000023677; RP1-164F3.2; truncated Bruton agammaglobulinemia tyrosine kinase; Tyrosine-protein kinase BTK; tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14)
Gene Aliases: AGMX1; AT; ATK; BPK; BTK; IMD1; PSCTK1; XLA
UniProt ID: (Human) Q06187
Entrez Gene ID: (Human) 695
negative regulation of cytokine production
adaptive immune response
regulation of B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
regulation of B cell apoptotic process
transcription, DNA-templated
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
I-kappaB kinase/NF-kappaB signaling
mesoderm development
peptidyl-tyrosine phosphorylation
calcium-mediated signaling
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
Fc-epsilon receptor signaling pathway
B cell activation
regulation of cell proliferation
innate immune response
positive regulation of B cell differentiation
cell maturation
B cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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