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Invitrogen
Phospho-CD150 (Tyr281) Polyclonal Antibody
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Phospho-CD150 (Tyr281) Antibody (PA5-106209) in IHC (P)
Immunohistochemistry analysis of Phospho-CD150 (Tyr281) in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-CD150 (Tyr281) polyclonal antibody (Product # PA5-106209) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-106209
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLAMF1(Accession Q13291), corresponding to amino acid residues around phosphorylated Tyr281.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817607
Product Specific Information
Antibody detects endogenous levels of SLAM / CD150 only when phosphorylated at Tyr281.
Target Information
CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD150; CD150 antigen; CDw150; IPO-3; signaling lymphocyte activation molecule; Signaling lymphocytic activation molecule; SLAM family member 1
Gene Aliases: 4933415F16; AA177906; CD150; CDw150; ESTM51; IPO-3; SLAM; SLAMF1
UniProt ID: (Human) Q13291, (Mouse) Q9QUM4
Entrez Gene ID: (Human) 6504, (Mouse) 27218
regulation of vesicle fusion
lymphocyte activation
regulation of catalytic activity
natural killer cell differentiation
natural killer cell proliferation
myeloid dendritic cell activation involved in immune response
negative regulation of T cell cytokine production
phagocytosis
cell adhesion
signal transduction
positive regulation of cell proliferation
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
T-helper 1 cell cytokine production
positive regulation of activated T cell proliferation
innate immune response
positive regulation of JNK cascade
viral entry into host cell
positive regulation of ERK1 and ERK2 cascade
negative regulation of interferon-gamma secretion
positive regulation of interferon-gamma secretion
negative regulation of CD40 signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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