Invitrogen

Phospho-CDK1/CDK2/CDK3 (Thr14) Recombinant Rabbit Monoclonal Antibody (4I9L3)

View all (2) CDK1/CDK2/CDK3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-35888

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4I9L3

Immunogen

A synthetic phosphorylated peptide around T14 of human CDK1/2/3CDK1 (P06493)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849786

Product Specific Information

Immunogen sequence: EGTYG

Target Information

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1- mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1- dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the 2 centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis (PubMed:26549230).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDK1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; cell division cycle 2 homolog A; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cell division protein kinase 2; Cell division protein kinase 3; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 1; Cyclin-dependent kinase 2; Cyclin-dependent kinase 3; p33 protein kinase; p34 protein kinase

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Gene Aliases: 2310050O10Rik; A630093N05Rik; CDC2; CDC28A; Cdc2a; CDK1; CDK2; CDK3; CDKN1; CDKN2; CDKN3; p33(CDK2); p34; P34CDC2

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UniProt ID: (Human) P06493, (Human) P24941, (Human) Q00526, (Rat) P39951, (Mouse) P11440, (Mouse) P97377

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Entrez Gene ID: (Human) 983, (Human) 1017, (Human) 1018, (Rat) 54237, (Mouse) 12534, (Rat) 362817, (Mouse) 12566, (Mouse) 69681

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Function(s)
protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity cyclin binding Hsp70 protein binding histone kinase activity kinase activity protein complex binding metal ion binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
mitotic cell cycle protein complex assembly protein phosphorylation apoptotic process mitotic nuclear division mitotic G2 DNA damage checkpoint cell aging response to toxic substance response to organonitrogen compound positive regulation of gene expression response to organic cyclic compound response to amine response to activity histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation organ regeneration positive regulation of protein import into nucleus, translocation protein localization to kinetochore response to drug response to hydrogen peroxide negative regulation of apoptotic process response to ethanol positive regulation of DNA replication positive regulation of mitotic cell cycle response to cadmium ion response to copper ion response to axon injury cell division ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation cellular response to hydrogen peroxide G1/S transition of mitotic cell cycle lens development in camera-type eye DNA repair potassium ion transport Ras protein signal transduction positive regulation of cell proliferation positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus positive regulation of transcription, DNA-templated meiotic cell cycle response to cAMP response to electrical stimulus regulation of cell cycle regulation of gene silencing cell cycle phosphorylation cellular response to DNA damage stimulus cell proliferation G0 to G1 transition G2/M transition of mitotic cell cycle DNA replication DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest centriole replication meiotic nuclear division mitotic G1 DNA damage checkpoint centrosome duplication negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle cellular response to nitric oxide regulation of signal transduction by p53 class mediator regulation of transcription involved in G1/S transition of mitotic cell cycle activation of MAPK activity microtubule cytoskeleton organization mitotic nuclear envelope disassembly centrosome cycle pronuclear fusion regulation of Schwann cell differentiation cell migration anaphase-promoting complex-dependent catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of embryonic development positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Golgi disassembly positive regulation of protein localization to nucleus
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