Invitrogen

Phospho-CHK1 (Ser345) Polyclonal Antibody

Phospho-CHK1 (Ser345) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-CHK1 (Ser345) Antibody (PA5-34625) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Phospho-CHK1 (Ser345) using Phospho-CHK1 (Ser345) Polyclonal Antibody (Product # PA5-34625) (Green) diluted at 1:500. HeLa cells mock (left) and treated with 100 J/m2 UVC and recover for 8 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Phospho-CHK1 (Ser345) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-CHK1 (Ser345) Antibody in Western Blot (WB)

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Product Details

PA5-34625

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser345 of human Chk1. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.66 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2551977

Product Specific Information

Recommended positive controls: HCT116, HCT116 cells with 30uM cisplatin treatment for 24hr.

Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%).

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibiting WEE1 Augments the Antitumor Efficacy of Cisplatin in Urothelial Carcinoma by Enhancing the DNA Damage Process.

Cells

Su YL,Xiao LY,Huang SY,Wu CC,Chang LC,Chen YH,Luo HL,Huang CC,Liu TT,Peng JM

PA5-34625 was used in Western Blot to investigate the antitumor efficacy of the Wee1 inhibitor (AZD-1775) alone or in combination with cisplatin was evaluated in urothelial carcinoma (UC) cell lines and a xenograft mouse model.

Thu May 25 00:00:00 UTC 2023

The Ataxia telangiectasia-mutated and Rad3-related protein kinase regulates cellular hydrogen sulfide concentrations.

DNA repair

Chen J,Shen X,Pardue S,Meram AT,Rajendran S,Ghali GE,Kevil CG,Shackelford RE

Published figure using Phospho-CHK1 (Ser345) polyclonal antibody (Product # PA5-34625) in Western Blot

Tue Jan 01 00:00:00 UTC 2019

The ATM and Rad3-Related (ATR) Protein Kinase Pathway Is Activated by Herpes Simplex Virus 1 and Required for Efficient Viral Replication.

Journal of virology

Edwards TG,Bloom DC,Fisher C

Published figure using Phospho-CHK1 (Ser345) polyclonal antibody (Product # PA5-34625) in Immunofluorescence

Thu Mar 15 00:00:00 UTC 2018

Elevated PTTG and PBF predicts poor patient outcome and modulates DNA damage response genes in thyroid cancer.

Oncogene

Read ML,Fong JC,Modasia B,Fletcher A,Imruetaicharoenchoke W,Thompson RJ,Nieto H,Reynolds JJ,Bacon A,Mallick U,Hackshaw A,Watkinson JC,Boelaert K,Turnell AS,Smith VE,McCabe CJ

PA5-34625 was used in Western Blot to characterise the combined role of PTTG and PBF in tumourigenesis.

Thu Sep 14 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1; Synoviolin

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Gene Aliases: C85740; CHEK1; CHK1; rad27

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UniProt ID: (Human) O14757, (Mouse) O35280

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Entrez Gene ID: (Human) 1111, (Mouse) 12649

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity histone kinase activity (H3-T11 specific) nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

DNA damage checkpoint G2/M transition of mitotic cell cycle DNA repair protein phosphorylation cellular response to DNA damage stimulus DNA damage induced protein phosphorylation cell cycle regulation of gene expression regulation of double-strand break repair via homologous recombination regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage negative regulation of G2/M transition of mitotic cell cycle phosphorylation peptidyl-threonine phosphorylation G2 DNA damage checkpoint histone H3-T11 phosphorylation regulation of cell proliferation negative regulation of mitotic nuclear division regulation of mitotic centrosome separation chromatin-mediated maintenance of transcription negative regulation of DNA biosynthetic process regulation of histone H3-K9 acetylation DNA replication cellular response to mechanical stimulus replicative senescence regulation of signal transduction by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-CHK1 (Ser345) Polyclonal Antibody

Phospho-CHK1 (Ser345) Antibody (PA5-34625) in ICC/IF

Product Details

PA5-34625

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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