Invitrogen

Phospho-CHK2 (Thr68) Monoclonal Antibody (ebchk2), PE, eBioscience™

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

12-9508-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
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Miscellaneous PubMed (MISC)

-
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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ebchk2

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572718

Product Specific Information

Description: This ebchk2 monoclonal antibody recognizes human and mouse Checkpoint Kinase 2 (Chk2) when phosphorylated on threonine 68 (T68). Chk2 is a cell cycle checkpoint regulator and tumor suppressor. Along with Chk1, it plays a critical role in the cellular response to DNA damage, and is particularly important during G2/M transition. Chk2 is activated via phosphorylation on T68 by the kinase ATM, which responds primarily to double-stranded DNA breaks. Upon activation, Chk2 functions include inhibition of Cdc25 family phosphatases and activation of p53, both of which prevent progression of the cell cycle. Abnormal regulation of the ATM-Chk2 pathway is often observed in cancer.

Applications Reported:This ebchk2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This ebchk2 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CHEK2 (CHK2) is a serine/threonine kinase and a component of the DNA damage checkpoint pathway. A mutation in CHK2 has been linked to cancer. CHK2 is activated by DNA damage and phosphorylates several modulators of cell cycle control including tumor suppressor proteins. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53. Also, mutations in CHK2 are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; checkpoint-like protein CHK2; Chk2 (phospho T68); Chk2 (pThr68); CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); Chk2 phospho; Chk2 phospho T68; del9; EC 2.7.11.1; HuCds 1; Hucds1; kinase Chk2; OTTHUMP00000199044; OTTHUMP00000199045; OTTHUMP00000199064; OTTHUMP00000199115; OTTHUMP00000199116; phospho Chk2 (T68); phospho Thr68 Chk2; protein kinase Chk2; Rad53 homolog; RP11-436C9.1; Serine/threonine protein kinase Chk2; Serine/threonine-protein kinase Chk2

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Gene Aliases: CDS1; CHEK2; CHK2; hCds1; HUCDS1; LFS2; PP1425; RAD53

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UniProt ID: (Human) O96017, (Mouse) Q9Z265

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Entrez Gene ID: (Human) 11200, (Mouse) 50883

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Function(s)
protein serine/threonine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity metal ion binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
DNA damage checkpoint G2/M transition of mitotic cell cycle replicative cell aging DNA repair double-strand break repair transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation apoptotic process cellular response to DNA damage stimulus DNA damage induced protein phosphorylation cell cycle mitotic nuclear division intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation phosphorylation regulation of protein catabolic process signal transduction in response to DNA damage cellular protein catabolic process positive regulation of transcription, DNA-templated protein autophosphorylation protein stabilization cell division signal transduction involved in intra-S DNA damage checkpoint mitotic spindle assembly positive regulation of protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest peptidyl-serine phosphorylation cellular response to drug negative regulation of cell cycle arrest replicative senescence regulation of signal transduction by p53 class mediator response to doxorubicin cellular response to bisphenol A negative regulation of DNA damage checkpoint positive regulation of anoikis
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