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Invitrogen
Phospho-CK1 alpha (Tyr294) Polyclonal Antibody
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Phospho-CK1 alpha (Tyr294) Antibody (PA5-103266) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-CK1 alpha (Tyr294) in rat heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-CK1 alpha (Tyr294) polyclonal antibody (Product # PA5-103266) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-103266
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CSNK1A1(Accession P48729), corresponding to amino acid residues around phosphorylated Tyr294.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815965
Product Specific Information
Antibody detects endogenous levels of Casein Kinase 1 alpha only when phosphorylated at PhoTyr294.
Target Information
CNSK1A1 (CK1 alpha 1) is a member of the casein kinase I (CKI) gene family, which encodes serine/threonine kinases that preferentially phosphorylate acidic substrates. CKI proteins are monomeric, ranging from 25 to 55 kD, and are found in the nuclei, cytoplasm, and membrane fractions of eukaryotic cells. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: casein kinase 1, alpha 1; Casein kinase I isoform alpha; casein kinase I-alpha; CK I alpha; CK-I alpha; CK1; CKI alpha; CKI-alpha; clock regulator kinase; CSNK1A1/CK1; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; OTTHUMP00000224000; OTTHUMP00000224001; OTTHUMP00000224003; YIL035C
Gene Aliases: 2610208K14Rik; 4632404G05Rik; 5430427P18Rik; CK1; CK1a; CKIa; Csnk1a; CSNK1A1; HEL-S-77p; HLCDGP1; PRO2975
UniProt ID: (Human) P48729, (Rat) P97633
Entrez Gene ID: (Human) 1452, (Rat) 113927, (Mouse) 93687
cell morphogenesis
protein phosphorylation
phagocytosis
mitotic nuclear division
regulation of cell shape
Wnt signaling pathway
peptidyl-serine phosphorylation
cell division
Golgi organization
signal transduction
cell surface receptor signaling pathway
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
intermediate filament cytoskeleton organization
negative regulation of canonical Wnt signaling pathway
regulation of GTP binding
beta-catenin destruction complex assembly
beta-catenin destruction complex disassembly
cell cycle
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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