Invitrogen

Phospho-CaMKII beta/gamma/delta (Thr287) Polyclonal Antibody

Phospho-CaMKII beta/gamma/delta (Thr287) Antibody in Western Blot (WB)

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Phospho-CaMKII beta/gamma/delta (Thr287) Antibody (PA5-37833) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of SK-N-AS (Lane 1), SK-N-AS treated with Ionomycin (100 nM, for 24 hr) (Lane 2), tissue extracts of Mouse Brain (Lane 3) and Rat Brain (Lane 4). The blot was probed with Phospho-CaMKII beta/gamma/delta (Thr287) Polyclonal Antibody (Product # PA5-37833, 1µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain)... View More

Phospho-CaMKII beta/gamma/delta (Thr287) Antibody

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Product Details

PA5-37833

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of Threonine 287 (Q-E-T(p)-V-E) derived from Human CaMK2- beta/ gamma/ delta

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2554441

Product Specific Information

A suggested positive control for Western blot is rat brain cells.

Target Information

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated alpha, beta, gamma and delta, which may or may not be coexpressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.

Molecular therapy. Nucleic acids

Shiba N,Yang X,Sato M,Kadota S,Suzuki Y,Agata M,Nagamine K,Izumi M,Honda Y,Koganehira T,Kobayashi H,Ichimura H,Chuma S,Nakai J,Tohyama S,Fukuda K,Miyazaki D,Nakamura A,Shiba Y

PA5-37833 was used in Western Blot to compare three isogenic human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) expressing Δ3-9, frameshifting Δ3-7, or intact Duchenne muscular dystrophy (DMD).

Tue Dec 12 00:00:00 UTC 2023

Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.

Molecular therapy. Nucleic acids

Shiba N,Yang X,Sato M,Kadota S,Suzuki Y,Agata M,Nagamine K,Izumi M,Honda Y,Koganehira T,Kobayashi H,Ichimura H,Chuma S,Nakai J,Tohyama S,Fukuda K,Miyazaki D,Nakamura A,Shiba Y

Tue Dec 12 00:00:00 UTC 2023

TRPV1 Modulator Ameliorates Alzheimer-Like Amyloid-β Neuropathology via Akt/Gsk3β-Mediated Nrf2 Activation in the Neuro-2a/APP Cell Model.

Oxidative medicine and cellular longevity

Wang X,Bian Y,Wong CTT,Lu JH,Lee SM

PA5-37833 was used in Sample Preparation to investigate the neuroprotective potential of PcActx peptide and its underlying mechanism of action, in an N2a/APP cell model of AD. Both Western blot and RT-PCR analysis revealed that PcActx peptide markedly inhibited the production of amyloid-related proteins and the expression of BACE1, PSEN1, and PSEN2.

Mon Dec 12 00:00:00 UTC 2022

TRPV1 Modulator Ameliorates Alzheimer-Like Amyloid-β Neuropathology via Akt/Gsk3β-Mediated Nrf2 Activation in the Neuro-2a/APP Cell Model.

Oxidative medicine and cellular longevity

Wang X,Bian Y,Wong CTT,Lu JH,Lee SM

Mon Dec 12 00:00:00 UTC 2022

Synergistic Anticancer Effect of a Combination of Berbamine and Arcyriaflavin A against Glioblastoma Stem-like Cells.

Molecules (Basel, Switzerland)

Han JM,Jung HJ

PA5-37833 was used in Western Blotting to demonstrate a novel combination therapy involving berbamine and ArcA effectively eradicating GSCs.

Thu Nov 17 00:00:00 UTC 2022

Dapagliflozin reduces the vulnerability of rats with pulmonary arterial hypertension-induced right heart failure to ventricular arrhythmia by restoring calcium handling.

Cardiovascular diabetology

Wu J,Liu T,Shi S,Fan Z,Hiram R,Xiong F,Cui B,Su X,Chang R,Zhang W,Yan M,Tang Y,Huang H,Wu G,Huang C

PA5-37833 was used in Western Blotting to examine the effects of DAPA on VA vulnerability in a rat model of PAH-induced RHF.

Wed Sep 28 00:00:00 UTC 2022

Pyridostigmine improves cardiac function and rhythmicity through RyR2 stabilization and inhibition of STIM1-mediated calcium entry in heart failure.

Journal of cellular and molecular medicine

Baine S,Bonilla I,Belevych A,Stepanov A,Dorn LE,Terentyeva R,Terentyev D,Accornero F,Carnes CA,Gyorke S

PA5-37833 was used in Western Blotting to examine the effects of chronic treatment with the cholinesterase inhibitor pyridostigmine in pressure overload heart failure.

Sat May 01 00:00:00 UTC 2021

Pyridostigmine improves cardiac function and rhythmicity through RyR2 stabilization and inhibition of STIM1-mediated calcium entry in heart failure.

Journal of cellular and molecular medicine

Baine S,Bonilla I,Belevych A,Stepanov A,Dorn LE,Terentyeva R,Terentyev D,Accornero F,Carnes CA,Gyorke S

PA5-37833 was used in Western Blotting to examine the effects of chronic treatment with the cholinesterase inhibitor pyridostigmine in pressure overload heart failure.

Sat May 01 00:00:00 UTC 2021

Phosphoproteomics identify arachidonic-acid-regulated signal transduction pathways modulating macrophage functions with implications for ovarian cancer.

Theranostics

Dietze R,Hammoud MK,Gómez-Serrano M,Unger A,Bieringer T,Finkernagel F,Sokol AM,Nist A,Stiewe T,Reinartz S,Ponath V,Preußer C,von Strandmann EP,Müller-Brüsselbach S,Graumann J,Müller R

Published figure using Phospho-CaMKII beta/gamma/delta (Thr287) polyclonal antibody (Product # PA5-37833) in Western Blot

Thu Jul 22 00:00:00 UTC 2021

The Ca²⁺-activated cation channel TRPM4 is a positive regulator of pressure overload-induced cardiac hypertrophy.

eLife

Guo Y,Yu ZY,Wu J,Gong H,Kesteven S,Iismaa SE,Chan AY,Holman S,Pinto S,Pironet A,Cox CD,Graham RM,Vennekens R,Feneley MP,Martinac B

PA5-37833 was used in Western Blot to investigate if transient receptor potential melastatin 4 channel is an important component of the mechanosensory signalling pathway that induces left ventricular hypertrophy (LVH) in response to pressure overload and represents a potential novel therapeutic target for the prevention of pathological LVH.

Wed Jun 30 00:00:00 UTC 2021

Actionable Cytopathogenic Host Responses of Human Alveolar Type 2 Cells to SARS-CoV-2.

Molecular cell

Hekman RM,Hume AJ,Goel RK,Abo KM,Huang J,Blum BC,Werder RB,Suder EL,Paul I,Phanse S,Youssef A,Alysandratos KD,Padhorny D,Ojha S,Mora-Martin A,Kretov D,Ash PEA,Verma M,Zhao J,Patten JJ,Villacorta-Martin C,Bolzan D,Perea-Resa C,Bullitt E,Hinds A,Tilston-Lunel A,Varelas X,Farhangmehr S,Braunschweig U,Kwan JH,McComb M,Basu A,Saeed M,Perissi V,Burks EJ,Layne MD,Connor JH,Davey R,Cheng JX,Wolozin BL,Blencowe BJ,Wuchty S,Lyons SM,Kozakov D,Cifuentes D,Blower M,Kotton DN,Wilson AA,Mühlberger E,Emili A

PA5-37833 was used in Functional network inference to perform a quantitative phosphoproteomic survey of induced pluripotent stem cell-derived AT2s infected with SARS-CoV-2 at air-liquid interface, to understand the mechanisms driving infection and pathology.

Thu Dec 17 00:00:00 UTC 2020

Modifications of Sarcoplasmic Reticulum Function Prevent Progression of Sarcomere-Linked Hypertrophic Cardiomyopathy Despite a Persistent Increase in Myofilament Calcium Response.

Frontiers in physiology

Chowdhury SAK,Warren CM,Simon JN,Ryba DM,Batra A,Varga P,Kranias EG,Tardiff JC,Solaro RJ,Wolska BM

PA5-37833 was used in Western Blot to investigate a mouse model expressing the mutant cTnT-R92Q, which is linked to HCM and induces an increase in myofilament Ca2+ sensitivity and diastolic dysfunction.

Mon Sep 28 00:00:00 UTC 2020

Modifications of Sarcoplasmic Reticulum Function Prevent Progression of Sarcomere-Linked Hypertrophic Cardiomyopathy Despite a Persistent Increase in Myofilament Calcium Response.

Frontiers in physiology

Chowdhury SAK,Warren CM,Simon JN,Ryba DM,Batra A,Varga P,Kranias EG,Tardiff JC,Solaro RJ,Wolska BM

Mon Sep 28 00:00:00 UTC 2020

Activation of CaMKII via ER-stress mediates coxsackievirus B3-induced cardiomyocyte apoptosis.

Cell biology international

Nie J,Ta N,Liu L,Shi G,Kang T,Zheng Z

PA5-37833 was used in Flow Cytometry, Western Blot to show that CVB3-induced endoplasmic reticulum (ER) stress response and apoptosis in cultured H9c2 cardiomyocytes.

Sat Feb 01 00:00:00 UTC 2020

SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.

Frontiers in synaptic neuroscience

Shrestha A,Sultana R,Lee CC,Ogundele OM

Published figure using Phospho-CaMKII beta/gamma/delta (Thr287) polyclonal antibody (Product # PA5-37833) in Western Blot

Sun Apr 21 00:00:00 UTC 2024

CaMKII Activation Promotes Cardiac Electrical Remodeling and Increases the Susceptibility to Arrhythmia Induction in High-fat Diet-Fed Mice With Hyperlipidemia Conditions.

Journal of cardiovascular pharmacology

Zhong P,Quan D,Huang Y,Huang H

Published figure using Phospho-CaMKII beta/gamma/delta (Thr287) polyclonal antibody (Product # PA5-37833) in Western Blot

Sun Oct 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Ca++/calmodulin-dependent protein kinase 2 delta subunit; Ca++/calmodulin-dependent protein kinase II beta subunit; Ca++/calmodulin-dependent protein kinase II delta subunit; Ca++/calmodulin-dependent protein kinase II, delta subunit; Ca2+/calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase II gamma; calcium/calmodulin-dependent protein kinase type II beta chain; calcium/calmodulin-dependent protein kinase type II delta chain; calcium/calmodulin-dependent protein kinase type II gamma chain; Calcium/calmodulin-dependent protein kinase type II subunit beta; Calcium/calmodulin-dependent protein kinase type II subunit delta; Calcium/calmodulin-dependent protein kinase type II subunit gamma; CaM kinase II beta subunit; CaM kinase II delta subunit; CaM kinase II subunit beta; CaM kinase II subunit delta; CaM kinase II subunit gamma; CaM-kinase II beta chain; CaM-kinase II delta chain; caM-kinase II gamma chain; CaMK II; CaMK-II delta subunit; caMK-II subunit beta; caMK-II subunit delta; caMK-II subunit gamma; proline rich calmodulin-dependent protein kinase

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Gene Aliases: 2810011D23Rik; 8030469K03Rik; [d]-CaMKII; CAM2; CAMK; CaMK II; CAMK-II; CAMK1; CAMK2; CAMK2B; CAMK2D; CAMK2G; CAMKB; CAMKD; CAMKG; Camki; Ck2b; Kiaa4163; RATCAMKI

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UniProt ID: (Human) Q13554, (Human) Q13557, (Human) Q13555, (Rat) P08413, (Mouse) P28652, (Rat) P15791, (Mouse) Q6PHZ2, (Mouse) Q923T9, (Rat) P11730

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Entrez Gene ID: (Human) 816, (Human) 817, (Human) 818, (Rat) 24245, (Mouse) 12323, (Rat) 24246, (Mouse) 108058, (Mouse) 12325, (Rat) 171140

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Function(s)

actin binding protein serine/threonine kinase activity calmodulin-dependent protein kinase activity Ras guanyl-nucleotide exchange factor activity protein binding calmodulin binding ATP binding protein kinase binding protein homodimerization activity phospholipase binding sodium channel inhibitor activity titin binding ion channel binding nitric-oxide synthase binding calcium-dependent protein serine/threonine phosphatase activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

Performance Guarantee

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Phospho-CaMKII beta/gamma/delta (Thr287) Polyclonal Antibody

Phospho-CaMKII beta/gamma/delta (Thr287) Antibody (PA5-37833) in WB

Product Details

PA5-37833

Western Blot (WB)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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