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Phospho-Cdc25A (Ser18) Antibody (710837) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized HeLa cells treated with UV (302nM) for 60 minutes for detection of Cdc25apS18 using Anti-Cdc25apS18 Recombinant Rabbit Superclonal™ Antibody (Product # 710837, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that ... View More
Product Details
710837
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
20HCLC
Immunogen
Peptide corresponding to Human CDC25A (aa 14-21)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2609005
Product Specific Information
This antibody is predicted to react with Monkey and Bovine.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC25 isoform A1-CAG; CDC25A2-CAG isoform; Dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1; MPIP1
Gene Aliases: CDC25A; CDC25A2
UniProt ID: (Human) P30304
Entrez Gene ID: (Human) 993
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
DNA replication
mitotic nuclear division
cell proliferation
response to radiation
cellular response to UV
peptidyl-tyrosine dephosphorylation
cell division
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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