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Invitrogen
Phospho-Chk1 (Ser345) Recombinant Rabbit Monoclonal Antibody (Chk1S345-R3F9)
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FIGURE: 1 / 4
Phospho-Chk1 (Ser345) Antibody (MA5-37019) in Flow
Flow cytometry of Phospho-Chk1 (Ser345) using HeLa cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), untreated (red), treated with calyculin A (green), untreated and blocked with phospho-peptide (black), CalA and blocked with phospho peptide (gold), un... View More
Product Details
MA5-37019
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
Chk1S345-R3F9
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser345 of human phospho Chk1.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896954
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1; Synoviolin
Gene Aliases: C85740; CHEK1; CHK1; rad27
UniProt ID: (Human) O14757, (Rat) Q91ZN7, (Mouse) O35280
Entrez Gene ID: (Human) 1111, (Rat) 140583, (Mouse) 12649
DNA damage checkpoint
G2/M transition of mitotic cell cycle
DNA repair
protein phosphorylation
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
regulation of gene expression
regulation of double-strand break repair via homologous recombination
regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage
negative regulation of G2/M transition of mitotic cell cycle
peptidyl-threonine phosphorylation
histone H3-T11 phosphorylation
regulation of cell proliferation
negative regulation of mitotic nuclear division
regulation of mitotic centrosome separation
chromatin-mediated maintenance of transcription
cellular response to mechanical stimulus
cellular response to organic substance
cellular response to caffeine
negative regulation of DNA biosynthetic process
regulation of histone H3-K9 acetylation
cell cycle
phosphorylation
G2 DNA damage checkpoint
DNA replication
replicative senescence
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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