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Invitrogen
Phospho-Coronin 1B (Ser2) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Coronin 1B (Ser2) Antibody (PA5-143670) in ICC/IF
Immunocytochemistry analysis of Phospho-Coronin 1B (Ser2) in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal Coronin-1B (C-terminus) and Phospho-Coronin 1B (Ser2) polyclonal antibody (Product # PA5-143670), then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respe... View More
Product Details
PA5-143670
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Coronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this sequence has low homology to the conserved region in other coronins.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942898
Product Specific Information
The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment.
Target Information
Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: coronin 1b; coronin 2; coronin, actin binding protein, 1B; Coronin-1B; Coronin-2
Gene Aliases: CORO1B; CORONIN-2
UniProt ID: (Human) Q9BR76, (Rat) O89046, (Mouse) Q9WUM3
Entrez Gene ID: (Human) 57175, (Rat) 29474, (Mouse) 23789
cell migration
actin cytoskeleton organization
ruffle organization
negative regulation of Arp2/3 complex-mediated actin nucleation
endothelial cell chemotaxis
wound healing
actin filament bundle assembly
actin filament branching
protein localization to cell leading edge
positive regulation of lamellipodium morphogenesis
cellular response to platelet-derived growth factor stimulus
negative regulation of smooth muscle cell chemotaxis
negative regulation of lamellipodium morphogenesis
regulation of Arp2/3 complex-mediated actin nucleation
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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