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Invitrogen
Phospho-Cortactin (Tyr466) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-Cortactin (Tyr466) Antibody (44-856) in WB
Extracts of NIH3T3 cells stably expressing c-Src Y527F mutant protein were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was left untreated (1-4) or treated with lambda (λ) phosphatase (5), blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with the cortactin (pY466) (mouse) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following p... View More
Product Details
44-856
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
antiserum was produced against a chemically synthesized phosphopeptide derived from the region of mouse cortactin that contains tyrosine 466 (tyrosine 470 in the human sequence).
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533781
Target Information
Cortactin (Cttn) is a ubiquitous actin-binding protein that was originally identified as a substrate for Src. It contributes to the organization of the actin cytoskeleton and cell shape. Cortactin also plays a role in the formation of lamellipodia and cell migration. It is accumulated in peripheral, actin-enriched structures of cells suggesting that cortactin facilitates actin network formation. Cortactin has four major domains of interest the N-terminal acidic (NTA) and tandem repeats domains and the C-terminal proline-rich and SH3 Domains. NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin is involved in promoting cell motility and invasion, including a critical role in invadopodia, actin rich-subcellular protrusions associated with degradation of the ECM by cancer cells. Cortactin is phosphorylated by src family kinases at Y421, Y466, and Y482 and S405 and S418 that are phosphorylated by Erk family kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ34459; mammary tumor and squamous cell carcinoma associated (p80/85 src substrate); Oncogene SRC8; Src substrate cortactin; src8
Gene Aliases: 1110020L01Rik; Cttn; Ems1
UniProt ID: (Mouse) Q60598
Entrez Gene ID: (Mouse) 13043
intracellular protein transport
endocytosis
receptor-mediated endocytosis
substrate-dependent cell migration, cell extension
actin filament polymerization
regulation of axon extension
positive regulation of actin filament polymerization
actin cytoskeleton reorganization
positive regulation of smooth muscle contraction
focal adhesion assembly
neuron projection morphogenesis
cell motility
dendritic spine maintenance
lamellipodium organization
regulation of mitophagy
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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