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Invitrogen
Phospho-DARPP-32 (Thr75) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-DARPP-32 (Thr75) Antibody (PA1-4611) in WB
Western blot of DARPP-32 in rat striatal lysate showing specific immunolabeling of a band at ~32 kDa corresponding to Phospho-DARPP-32 (Thr75) polyclonal antibody (Product # PA1-4611) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).
Product Details
PA1-4611
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 of rat PPP1R1B conjugated to KLH
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_560609
Product Specific Information
This antibody is predicted to react with bovine, canine, chicken, human, mouse and non-human primate based on 100% sequence homology.
In Western blot, this antibody detects an ~32 kDa protein representing the Phospho-Thr75 DARPP-32 protein.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; PPR1B; protein pho; Protein phosphatase 1 regulatory subunit 1B
Gene Aliases: AU040756; DARPP-32; Darpp32; Ppp1r1b
UniProt ID: (Rat) Q6J4I0, (Mouse) Q60829
Entrez Gene ID: (Rat) 360616, (Mouse) 19049
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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