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Invitrogen
Phospho-DENND3 (Thr450) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-DENND3 (Thr450) Antibody (PA5-95667) in WB
Western blot of DENND3 in HeLa cell lysate showing specific immunolabeling of a band at ~142 kDa corresponding to Phospho-DENND3 (Thr450) polyclonal antibody (Product # PA5-95667) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (800 units/1 mg protein for 30 min).
Product Details
PA5-95667
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr450 conjugated to KLH
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen Affinity Chromatography
Storage buffer
10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807469
Product Specific Information
Antigen Affinity Purified from Pooled Serum
Target Information
Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (PubMed:20937701). Regulates autophagy in response to starvation through RAB12 activation. Starvation leads to ULK1/2-dependent phosphorylation of Ser-472 and Ser-490, which in turn allows recruitment of 14-3-3 adapter proteins and leads to up-regulation of GEF activity towards RAB12. Also plays a role in protein transport from recycling endosomes to lysosomes, regulating, for instance, the degradation of the transferrin receptor and of the amino acid transporter PAT4 (PubMed:20937701). Starvation also induces phosphorylation at Tyr-858, which leads to up-regulated GEF activity and initiates autophagy. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DENN domain-containing protein 3; DENN/MADD domain containing 3
Gene Aliases: DENND3; KIAA0870
UniProt ID: (Human) A2RUS2
Entrez Gene ID: (Human) 22898
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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