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Phospho-DIAPH2 (Ser196) Antibody (PA5-143679) in WB
Western blot of Phospho-DIAPH2 (Ser196) in mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus) (lanes 1 & 2) and Phospho-DIAPH2 (Ser196) polyclonal antibody (Product # PA5-143679) (lanes 3 & 4).
Product Details
PA5-143679
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-mDia3 (Ser-196) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-196 in human mDia3 (Diap2). This peptide sequence is identical in rat and mouse mDia3, and the site is well conserved in human mDia1/Diap1 (Ser-171) and mDia2/Diap3 (Ser-202).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942907
Product Specific Information
The antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of mDia3 on SDS-PAGE immunoblots of human Jurkat treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. The antibody may also detect phosphorylated mDia1/Diap1 (Ser-171) and mDia2/Diap3 (Ser-202).
Target Information
The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: diaphanous homolog 2; diaphanous-related formin 2; Diaphanous-related formin-2; diaphorase-2; DRF2; FLJ11167; mDia3; mDia3 isoform; Protein diaphanous homolog 2; RP11-212B22.1
Gene Aliases: DIA; DIA2; Dia3; Diap2; DIAPH2; DRF2; E430022I22Rik; POF; POF2; RGD1564399
UniProt ID: (Human) O60879, (Mouse) O70566
Entrez Gene ID: (Human) 1730, (Mouse) 54004, (Rat) 317189
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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