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Phospho-ECT2 (Thr790) Antibody (600-401-935) in WB
Western blot using Rocklands affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. After S... View More
Product Details
600-401-935
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Chimpanzee,
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein at the pT790 residue. Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2. Additional experiments are underway to clarify antibody specificity. A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ect2 oncogene; epithelial cell transforming sequence 2 oncogene; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; MGC138291; Protein ECT2
Gene Aliases: AI528536; ARHGEF31; ECT2; mKIAA4037
UniProt ID: (Human) Q9H8V3, (Mouse) Q07139
Entrez Gene ID: (Human) 1894, (Dog) 488172, (Rat) 361921, (Mouse) 13605, (Zebrafish) 445406
cell morphogenesis
cytokinesis
protein transport
activation of protein kinase activity
positive regulation of cytokinesis
regulation of Rho protein signal transduction
intracellular signal transduction
positive regulation of protein import into nucleus
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of GTPase activity
positive regulation of neuron differentiation
regulation of protein kinase activity
regulation of small GTPase mediated signal transduction
protein homooligomerization
regulation of attachment of spindle microtubules to kinetochore
cellular response to hydrogen peroxide
bicellular tight junction assembly
cellular response to calcium ion
cellular response to ionizing radiation
activation of GTPase activity
cytokinetic process
regulation of cytokinesis, actomyosin contractile ring assembly
transport
cell cycle
signal transduction
nervous system development
cell differentiation
cell division
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