Invitrogen

Phospho-EEF2 (Thr56) Recombinant Rabbit Monoclonal Antibody (17H31L21)

Phospho-EEF2 (Thr56) Antibody in Western Blot (WB)

FIGURE: 1 / 2

Phospho-EEF2 (Thr56) Antibody (702808) in WB

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), T98G (Lane 2), 3T3-L1 (Lane 3), 3T3-L1 treated with Forskolin (100 µM for 60 mins) (Lane 4), Hep G2 (Lane 5), Hep G2 treated with Forskolin (100 µM for 60 mins) (Lane 6), NIH/3T3 (Lane 7) and NIH/3T3 treated with Forskolin (100 µM for 60 mins) (Lane 8). The blots were probed with Anti-EEF2 pT56 Recombinant Rabbit M... View More

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Product Details

702808

Applications

Tested Dilution

Publications

Western Blot (WB)

2.5 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Pig

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

17H31L21

Immunogen

Peptide corresponding to human Phospho-EEF2 (Thr56) [aa54-aa61]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2723465

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Cat, Bovine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intrarenal arteriosclerosis and telomere attrition associate with dysregulation of the cholesterol pathway.

Aging

De Vusser K,Winckelmans E,Martens D,Lerut E,Kuypers D,Nawrot T,Naesens M

702808 was used in ChIP assay to illustrate that the superpathway of cholesterol biosynthesis interacts with the previously published association between shorter telomere length and arteriosclerosis.

Thu Apr 30 00:00:00 UTC 2020

Gene Expression in Placentas From Nondiabetic Women Giving Birth to Large for Gestational Age Infants.

Reproductive sciences (Thousand Oaks, Calif.)

Ahlsson F,Åkerud H,Schijven D,Olivier J,Sundström-Poromaa I

702808 was used in ChIP Assay, Western Blot to compare placental gene expression between healthy, nondiabetic mothers (n = 22) giving birth to LGA infants and body mass index-matched mothers (n = 24) giving birth to appropriate for gestational age infants.

Thu Oct 01 00:00:00 UTC 2015

Characterization of proposed human B-1 cells reveals pre-plasmablast phenotype.

Blood

Covens K,Verbinnen B,Geukens N,Meyts I,Schuit F,Van Lommel L,Jacquemin M,Bossuyt X

702808 was used in Western Blotting to offer perspectives for the investigation of differentiation of B cells into antibody secreting cells.

Thu Jun 27 00:00:00 UTC 2013

Resistant starch induces catabolic but suppresses immune and cell division pathways and changes the microbiome in the proximal colon of male pigs.

The Journal of nutrition

Haenen D,Souza da Silva C,Zhang J,Koopmans SJ,Bosch G,Vervoort J,Gerrits WJ,Kemp B,Smidt H,Müller M,Hooiveld GJ

702808 was used in Western Blot, ChIP Assay to provide novel insights on effects of resistant starch (RS) in proximal colon and contributes to our understanding of a healthy diet.

Sun Dec 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: EF-2; Elongation factor 2; polypeptidyl-tRNA translocase

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Gene Aliases: EEF-2; EEF2; EF-2; EF2; SCA26

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UniProt ID: (Human) P13639, (Mouse) P58252

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Entrez Gene ID: (Human) 1938, (Mouse) 13629

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Function(s)

p53 binding translation elongation factor activity GTPase activity protein binding GTP binding 5S rRNA binding protein kinase binding ribosome binding poly(A) RNA binding actin filament binding cadherin binding involved in cell-cell adhesion nucleotide binding

Process(es)

hematopoietic progenitor cell differentiation translation translational elongation positive regulation of translation response to ischemia skeletal muscle contraction aging glial cell proliferation response to fluoxetine peptidyl-diphthamide biosynthetic process from peptidyl-histidine response to estradiol response to endoplasmic reticulum stress skeletal muscle cell differentiation response to hydrogen peroxide response to ethanol response to folic acid cell-cell adhesion cellular response to brain-derived neurotrophic factor stimulus positive regulation of cytoplasmic translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-EEF2 (Thr56) Recombinant Rabbit Monoclonal Antibody (17H31L21)

Phospho-EEF2 (Thr56) Antibody (702808) in WB

Product Details

702808

Western Blot (WB)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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