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Phospho-EGFR (Tyr1148) Antibody (710213) in ICC/IF
Immunofluorescence analysis of Phospho-EGFR (Tyr1148) was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-EGFR (Tyr1148) Recombinant Rabbit Superclonal™... View More
Product Details
710213
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
8HCLC
Immunogen
Phosphopeptide corresponding to amino acids 1145-1153 of human epidermal growth factor receptor
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532629
Product Specific Information
This antibody is predicted to react with equine, mouse, rabbit and rat based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone
Gene Aliases: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61
UniProt ID: (Human) P00533
Entrez Gene ID: (Human) 1956
chromatin binding
double-stranded DNA binding
MAP kinase kinase kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
transmembrane signaling receptor activity
epidermal growth factor-activated receptor activity
Ras guanyl-nucleotide exchange factor activity
protein binding
ATP binding
enzyme binding
protein phosphatase binding
nitric-oxide synthase regulator activity
ubiquitin protein ligase binding
identical protein binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
protein heterodimerization activity
actin filament binding
cadherin binding involved in cell-cell adhesion
MAPK cascade
activation of MAPKK activity
cell morphogenesis
ossification
embryonic placenta development
positive regulation of protein phosphorylation
hair follicle development
response to stress
signal transduction
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
activation of phospholipase C activity
salivary gland morphogenesis
learning or memory
cell proliferation
positive regulation of cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
single organismal cell-cell adhesion
peptidyl-tyrosine phosphorylation
cerebral cortex cell migration
positive regulation of cell growth
positive regulation of cell migration
positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle
positive regulation of catenin import into nucleus
ERBB2 signaling pathway
negative regulation of epidermal growth factor receptor signaling pathway
negative regulation of protein catabolic process
positive regulation of phosphorylation
activation of phospholipase A2 activity by calcium-mediated signaling
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of GTPase activity
positive regulation of nitric oxide biosynthetic process
positive regulation of DNA repair
positive regulation of DNA replication
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
digestive tract morphogenesis
positive regulation of epithelial cell proliferation
regulation of peptidyl-tyrosine phosphorylation
regulation of nitric-oxide synthase activity
protein insertion into membrane
positive regulation of protein kinase B signaling
morphogenesis of an epithelial fold
eyelid development in camera-type eye
response to UV-A
positive regulation of ERK1 and ERK2 cascade
cellular response to amino acid stimulus
cellular response to epidermal growth factor stimulus
cellular response to estradiol stimulus
positive regulation of production of miRNAs involved in gene silencing by miRNA
regulation of cell motility
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