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Phospho-EIF2S1 (Ser51) Polyclonal Antibody

Phospho-EIF2S1 (Ser51) Antibody in Western Blot (WB)

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Phospho-EIF2S1 (Ser51) Antibody (28740-1-AP) in WB

Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28740-1-AP (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:2000 incubated 48451 overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Product Details

28740-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

570 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage.

International immunopharmacology

Yu TT,Hu J,Li QR,Peng XC,Xu HZ,Han N,Li LG,Yang XX,Xu X,Yang ZY,Chen H,Chen X,Wang MF,Li TF

Wed Feb 01 00:00:00 UTC 2023

Knockdown of Rab7B, But Not of Rab7A, Which Antagonistically Regulates Oligodendroglial Cell Morphological Differentiation, Recovers Tunicamycin-Induced Defective Differentiation in FBD-102b Cells.

Journal of molecular neuroscience : MN

Fukushima N,Shirai R,Sato T,Nakamura S,Ochiai A,Miyamoto Y,Yamauchi J

Thu Jun 01 00:00:00 UTC 2023

Discovery of rafoxanide as a novel agent for the treatment of non-small cell lung cancer.

Scientific reports

Hu A,Liu J,Wang Y,Zhang M,Guo Y,Qin Y,Liu T,Men Y,Chen Q,Liu T

Fri Jan 13 00:00:00 UTC 2023

G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration.

Cell proliferation

Li S,Liao Z,Yin H,Liu O,Hua W,Wu X,Zhang Y,Gao Y,Yang C

Wed Mar 01 00:00:00 UTC 2023

Novel neutrophil extracellular trap-related mechanisms in diabetic wounds inspire a promising treatment strategy with hypoxia-challenged small extracellular vesicles.

Bioactive materials

Chu Z,Huang Q,Ma K,Liu X,Zhang W,Cui S,Wei Q,Gao H,Hu W,Wang Z,Meng S,Tian L,Li H,Fu X,Zhang C

Fri Sep 01 00:00:00 UTC 2023

Autophagosomes Defeat Ferroptosis by Decreasing Generation and Increasing Discharge of Free Fe²⁺ in Skin Repair Cells to Accelerate Diabetic Wound Healing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Cui S,Liu X,Liu Y,Hu W,Ma K,Huang Q,Chu Z,Tian L,Meng S,Su J,Zhang W,Li H,Fu X,Zhang C

Fri Sep 01 00:00:00 UTC 2023

African Swine Fever Virus MGF110-7L Induces Host Cell Translation Suppression and Stress Granule Formation by Activating the PERK/PKR-eIF2α Pathway.

Microbiology spectrum

Zhong H,Fan S,Du Y,Zhang Y,Zhang A,Jiang D,Han S,Wan B,Zhang G

Wed Dec 21 00:00:00 UTC 2022

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating.

Journal of materials chemistry. B

Yan B,Liu C,Wang S,Li H,Jiao J,Lee WSV,Zhang S,Hou Y,Hou Y,Ma X,Fan H,Lv Y,Liu X

Wed Jul 20 00:00:00 UTC 2022

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating.

Journal of materials chemistry. B

Yan B,Liu C,Wang S,Li H,Jiao J,Lee WSV,Zhang S,Hou Y,Hou Y,Ma X,Fan H,Lv Y,Liu X

Wed Jul 20 00:00:00 UTC 2022

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating.

Journal of materials chemistry. B

Yan B,Liu C,Wang S,Li H,Jiao J,Lee WSV,Zhang S,Hou Y,Hou Y,Ma X,Fan H,Lv Y,Liu X

Wed Jul 20 00:00:00 UTC 2022

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating.

Journal of materials chemistry. B

Yan B,Liu C,Wang S,Li H,Jiao J,Lee WSV,Zhang S,Hou Y,Hou Y,Ma X,Fan H,Lv Y,Liu X

Wed Jul 20 00:00:00 UTC 2022

CircZXDC Promotes Vascular Smooth Muscle Cell Transdifferentiation via Regulating miRNA-125a-3p/ABCC6 in Moyamoya Disease.

Cells

Liu Y,Huang Y,Zhang X,Ma X,He X,Gan C,Zou X,Wang S,Shu K,Lei T,Zhang H

Sat Nov 26 00:00:00 UTC 2022

Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.

Journal of molecular neuroscience : MN

Chang L,Liu X,Chen J,Liu H,Wang G,Wang G,Liao X,Shen X

Tue Mar 01 00:00:00 UTC 2022

The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.

Oxidative medicine and cellular longevity

Du Y,Zhong F,Cheng H,Li T,Chen Y,Tan P,Huang M,Liang T,Liu Y,Xia X,Fu W

Thu Feb 17 00:00:00 UTC 2022

Plasma Purification Treatment Relieves the Damage of Hyperlipidemia to PBMCs.

Frontiers in cardiovascular medicine

Zhang XM,Gu YH,Deng H,Xu ZQ,Zhong ZY,Lyu XJ,Jin HM,Yang XH

Tue Jul 27 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A

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Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1

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UniProt ID: (Human) P05198, (Rat) P68101, (Mouse) Q6ZWX6

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Entrez Gene ID: (Human) 1965, (Rat) 54318, (Mouse) 13665

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Function(s)

translation initiation factor activity protein binding ribosome binding poly(A) RNA binding nucleic acid binding RNA binding

Process(es)

translation translational initiation regulation of translational initiation in response to stress protein autophosphorylation positive regulation of defense response to virus by host regulation of translation mitophagy in response to mitochondrial depolarization aging negative regulation of translational initiation in response to stress cellular response to amino acid starvation cellular response to heat cellular response to UV response to endoplasmic reticulum stress PERK-mediated unfolded protein response transmembrane transport xenophagy positive regulation of neuron death response to manganese-induced endoplasmic reticulum stress positive regulation of type B pancreatic cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-EIF2S1 (Ser51) Polyclonal Antibody

Phospho-EIF2S1 (Ser51) Antibody (28740-1-AP) in WB

Product Details

28740-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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