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Invitrogen
Phospho-EIF2S1 (Ser51) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-EIF2S1 (Ser51) Antibody (PA5-121272) in WB
Western blot analysis of Phospho-EIF2S1 (Ser51) in extracts of various cell lines. Samples were incubated with Phospho-EIF2S1 (Ser51) Polyclonal antibody (Product # PA5-121272) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Lys... View More
Product Details
PA5-121272
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S51 of human EIF2S1 (NP_0040851)
Conjugate
Form
Liquid
Concentration
1.7275 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914844
Product Specific Information
Positive test controls include: HeLa, C6. The target is usually found in the following locations: Cytoplasmic granule.
Immunogen sequence: SELSR
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Gene Aliases: EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1
UniProt ID: (Human) P05198, (Rat) P68101
Entrez Gene ID: (Human) 1965, (Rat) 54318
translation
translational initiation
regulation of translational initiation in response to stress
protein autophosphorylation
positive regulation of defense response to virus by host
aging
negative regulation of translational initiation in response to stress
cellular response to amino acid starvation
cellular response to heat
cellular response to UV
response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
transmembrane transport
mitophagy in response to mitochondrial depolarization
xenophagy
positive regulation of neuron death
response to manganese-induced endoplasmic reticulum stress
positive regulation of type B pancreatic cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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