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Phospho-EIF2S1 (Ser51) Antibody (710292) in ICC/IF
Immunofluorescent analysis of Phospho-eIF2a pSer51 in HeLa cells using a Phospho-eIF2a pSer51 Recombinant Rabbit Superclonal™ Antibody (Product # 710292) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showin... View More
Product Details
710292
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
10HCLC
Immunogen
Phosphopeptide corresponding to amino acids 48-57 of human EIF2a [pS51]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532669
Product Specific Information
This antibody is predicted to react with non-human primate, rat, mouse, rabbit and equine based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-2-alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Gene Aliases: EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1
UniProt ID: (Human) P05198
Entrez Gene ID: (Human) 1965
positive regulation of defense response to virus by host
translational initiation
aging
negative regulation of translational initiation in response to stress
cellular response to amino acid starvation
cellular response to heat
cellular response to UV
response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
protein autophosphorylation
transmembrane transport
mitophagy in response to mitochondrial depolarization
xenophagy
positive regulation of neuron death
response to manganese-induced endoplasmic reticulum stress
positive regulation of type B pancreatic cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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