Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • EPRS Antibodies

          Invitrogen

          Phospho-EPRS (Tyr377) Polyclonal Antibody

          View all (17) EPRS antibodies

          Promotion

          View available promotion(s)

          Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Phospho-EPRS (Tyr377) Polyclonal Antibody

          • Antibody Testing Data (5)
          Phospho-EPRS (Tyr377) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-EPRS (Tyr377) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          Phospho-EPRS (Tyr377) Antibody (PA5-105476) in ICC/IF

          Immunocytochemistry analysis of Phospho-EPRS (Tyr377) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-EPRS (Tyr377) polyclonal antibody (Product # PA5-105476) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          Phospho-EPRS (Tyr377) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-EPRS (Tyr377) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-EPRS (Tyr377) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-EPRS (Tyr377) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-EPRS (Tyr377) Antibody in Western Blot (WB)

          Product Details

          PA5-105476

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human EPRS(Accession P07814), corresponding to amino acid residues around phosphorylated Tyr377.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          sequential chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice

          RRID

          AB_2816904

          Product Specific Information

          Antibody detects endogenous levels of EPRS only when phosphorylated at Tyr377.

          Target Information

          Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. EPRS is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline--tRNA ligase; Cell proliferation-inducing gene 32 protein; glutamate tRNA ligase; Glutamatyl-prolyl-tRNA synthetase; glutamine-proline-tRNA synthetase; glutaminyl-tRNA synthetase; proliferation-inducing gene 32 protein; proliferation-inducing protein 32; proline tRNA ligase; proline-tRNA ligase; prolyl-tRNA synthetase

          View more View less

          Gene Aliases: 2410081F06Rik; 3010002K18Rik; C79379; EARS; EPRS; EPRS1; GLNS; GLUPRORS; PARS; PIG32; QARS; QPRS

          View more View less

          UniProt ID: (Human) P07814, (Mouse) Q8CGC7

          View more View less

          Entrez Gene ID: (Human) 2058, (Mouse) 107508

          View more View less

          Function(s)
          glutamate-tRNA ligase activity proline-tRNA ligase activity protein binding ATP binding RNA stem-loop binding GTPase binding nucleotide binding RNA binding catalytic activity aminoacyl-tRNA ligase activity ligase activity ligase activity, forming aminoacyl-tRNA and related compounds
          Process(es)
          tRNA aminoacylation for protein translation glutamyl-tRNA aminoacylation prolyl-tRNA aminoacylation protein complex assembly negative regulation of translation cellular response to interferon-gamma translation regulation of translation metabolic process tRNA aminoacylation
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-67c77f6dc7-n6nf2:80/100.66.135.186:80.
          git-commit: 545e792480003146e539d624bd2a21b51d198228
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.0-2025.06.23-1.0