Invitrogen

Phospho-ERK1/ERK2 (Thr185, Thr202) Recombinant Rabbit Monoclonal Antibody (2D5)

View all (85) ERK1/2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-33179

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:20-1:200
-

ELISA (ELISA)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2D5

Immunogen

A synthesized peptide derived from human Phospho-MAPK3 (T202) + MAPK1 (T185)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.85 mg/mL

Purification

Protein G, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2811995

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; p42; p42 MAP Kinase; p42-MAPK; p44-ERK1; p44-MAPK; protein tyrosine kinase ERK2

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Gene Aliases: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; HS44KDAP; HUMKER1A; MAPK1; MAPK2; MAPK3; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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UniProt ID: (Human) P28482, (Human) P27361

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Entrez Gene ID: (Human) 5594, (Human) 5595

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Function(s)
phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding
Process(es)
MAPK cascade activation of MAPK activity MAPK import into nucleus positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly protein phosphorylation apoptotic process DNA damage induced protein phosphorylation cell cycle axon guidance fibroblast growth factor receptor signaling pathway response to toxic substance regulation of phosphatidylinositol 3-kinase signaling viral process phosphorylation peptidyl-serine phosphorylation sensory perception of pain arachidonic acid metabolic process platelet activation BMP signaling pathway thyroid gland development positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis outer ear morphogenesis response to exogenous dsRNA positive regulation of translation positive regulation of transcription from RNA polymerase II promoter thymus development regulation of sequence-specific DNA binding transcription factor activity cartilage development regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus caveolin-mediated endocytosis regulation of Golgi inheritance regulation of cellular response to heat positive regulation of telomere capping positive regulation of xenophagy regulation of early endosome to late endosome transport negative regulation of apolipoprotein binding transcription, DNA-templated chemotaxis response to stress cellular response to DNA damage stimulus signal transduction chemical synaptic transmission positive regulation of cell proliferation positive regulation of peptidyl-threonine phosphorylation peptidyl-threonine phosphorylation cytosine metabolic process positive regulation of cell migration regulation of protein stability mammary gland epithelial cell proliferation ERBB signaling pathway response to estrogen negative regulation of cell differentiation positive regulation of transcription, DNA-templated T cell receptor signaling pathway B cell receptor signaling pathway long-term synaptic potentiation labyrinthine layer blood vessel development cellular response to granulocyte macrophage colony-stimulating factor stimulus
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