Invitrogen

Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal Antibody

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (44-680G) in IHC (P)

Immunohistochemistry analysis of ERK1/2 (pTpY185/187) showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methan... View More

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunocytochemistry (ICC/IF)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (WB, ICC/IF, IHC)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody

Product Image Gallery

Product Details

44-680G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 56 publications 56 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 2 publications 2 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Miscellaneous PubMed (MISC)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Bovine, C. elegans, Chicken, Fruit fly, Human, Mouse, Rat, Xenopus

Published species

C. elegans, Dog, Human, Mouse, Pig, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533719

Product Specific Information

Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK1&2. The final product is generated by affinity chromatography using an ERK1&2-derived peptide that is phosphorylated at threonine 202/185 and tyrosine 204/187, respectively, within the activation loop.

This antibody has been used in western blotting and previous lots have been used in immunostaining. Other applications may work but have not been tested. The positive control used in western analysis were PC12 cells +/- Sorbitol; NIH3T3 +/- PDGF; and HEK293 +/- UV.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pinostrobin from Boesenbergia rotunda attenuates oxidative stress and promotes functional recovery in rat model of sciatic nerve crush injury.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Kongsui R,Surapinit S,Promsrisuk T,Thongrong S

44-680G was used in Immunohistochemistry-immunofluorescence to indicate that PB diminished the oxidative stress-induced nerve injury.

Thu Mar 02 00:00:00 UTC 2023

Insights into cellular behavior and micromolecular communication in urothelial micrografts.

Scientific reports

Juul N,Willacy O,Mamand DR,Andaloussi SE,Eisfeldt J,Chamorro CI,Fossum M

44-680G was used in Immunohistochemistry to demonstrate that increased tissue fragmentation subsequently potentiated the yield of proliferative cells and the cellular expansion potential, which confirms, that the micrografting principles of skin epithelium also apply to uroepithelium.

Mon Aug 21 00:00:00 UTC 2023

4-F-PCP, a Novel PCP Analog Ameliorates the Depressive-Like Behavior of Chronic Social Defeat Stress Mice via NMDA Receptor Antagonism.

Biomolecules & therapeutics

Ortiz DMD,Kim M,Lee HJ,Botanas CJ,Custodio RJP,Sayson LV,Campomayor NB,Lee C,Lee YS,Cheong JH,Kim HJ

44-680G was used in Immunohistochemistry (Paraffin), Western Blot to provide significant implications for future research not only with PCP analogs but also on the next generation of different types of antidepressants.

Wed Mar 01 00:00:00 UTC 2023

Sialylation of TLR2 initiates osteoclast fusion.

Bone research

Dou C,Zhen G,Dan Y,Wan M,Limjunyawong N,Cao X

44-680G was used in Western Blot to reveal that cell recognition mediated by the binding of sialylated TLR2 to Siglec15 initiates cell fusion for osteoclast formation.

Wed Mar 02 00:00:00 UTC 2022

Suppression of phosphodiesterase IV enzyme by roflumilast ameliorates cognitive dysfunction in aged rats after sevoflurane anaesthesia via PKA-CREB and MEK/ERK pathways.

The European journal of neuroscience

Liu P,Wang J,Peng S,Zhang D,Zhuang L,Liu C,Zhang Y,Shi X

44-680G was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that RF treatment promotes neuronal activity by maintaining cAMP levels and sustaining the activation of PKA-CREB and MEK/ERK pathways, offering novel insights for POCD therapy.

Mon Aug 01 00:00:00 UTC 2022

Inhibition of colon cancer K-Ras^G13D mutation reduces cancer cell proliferation but promotes stemness and inflammation via RAS/ERK pathway.

Frontiers in pharmacology

Qi Y,Zou H,Zhao X,Kapeleris J,Monteiro M,Li F,Xu ZP,Deng Y,Wu Y,Tang Y,Gu W

44-680G was used in Immunohistochemistry (Paraffin), Western Blot to suggest that K-RasG13D mutation promotes cancer cell growth but decreases cancer stemness and inflammation thus tumorigenesis and metastasis potential in colon cancer.

Fri Nov 18 00:00:00 UTC 2022

Inhibition of colon cancer K-Ras^G13D mutation reduces cancer cell proliferation but promotes stemness and inflammation via RAS/ERK pathway.

Frontiers in pharmacology

Qi Y,Zou H,Zhao X,Kapeleris J,Monteiro M,Li F,Xu ZP,Deng Y,Wu Y,Tang Y,Gu W

Fri Nov 18 00:00:00 UTC 2022

The Synergistic Effect of Cyclic Tensile Force and Periodontal Ligament Cell-Laden Calcium Silicate/Gelatin Methacrylate Auxetic Hydrogel Scaffolds for Bone Regeneration.

Cells

Lee JJ,Ng HY,Lin YH,Lin TJ,Kao CT,Shie MY

44-680G was used in Western Blotting to show that novel auxetic scaffolds could be fabricated by combining different aspects of science and technology, in order to improve the future chances of clinical applications for bone regeneration.

Wed Jun 29 00:00:00 UTC 2022

Andrographolide Relieves Post-Operative Wound Pain but Affects Local Angiogenesis.

Pharmaceuticals (Basel, Switzerland)

Lin YL,Liao JW,Wang S,Sridharan B,Lee HJ,Li A,Chang KM,Wu CY,Huang S,Chang KT,Agrawal DC,Chen CJ,Lee MJ

44-680G was used in Immunohistochemistry to explore the ability of Andro to reduce pain sensation in a rat post-operative wound model.

Mon Dec 19 00:00:00 UTC 2022

EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways.

Open medicine (Warsaw, Poland)

Ming D,Ma J

44-680G was used in Immunohistochemistry (Paraffin), Western Blot to conclude that miR-3666 downregulated EphA3 expression and retarded melanoma malignancy via inactivating ERK1/2 and p38 MAPK pathways.

Tue Jan 03 00:00:00 UTC 2023

Novel peanut-specific human IgE monoclonal antibodies enable screens for inhibitors of the effector phase in food allergy.

Frontiers in immunology

Suber J,Zhang Y,Ye P,Guo R,Burks AW,Kulis MD,Smith SA,Iweala OI

44-680G was used in Western Blotting to provide a unique screening tool to assess the efficacy of therapeutics that target allergy effector cells and inhibit food allergen-induced effector cell activation.

Tue Oct 18 00:00:00 UTC 2022

Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis.

Frontiers in cell and developmental biology

Roversi FM,Bueno MLP,Pericole FV,Saad STO

44-680G was used in Western Blot to study the role of one SFK member, the Hematopoietic Cell Kinase (HCK), in CXCL12/CXCR4 pathway and its contribution to the AML pathogenesis.

Tue Apr 13 00:00:00 UTC 2021

Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment.

Clinical oral investigations

Ruiz-Heiland G,Yong JW,von Bremen J,Ruf S

44-680G was used in Western Blotting to show that leptin influences the physiological effect of compressive forces on cementoblasts, exerting in vitro a proinflammatory and pro-apoptotic effect.

Thu Apr 01 00:00:00 UTC 2021

Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment.

Clinical oral investigations

Ruiz-Heiland G,Yong JW,von Bremen J,Ruf S

44-680G was used in Western Blotting to show that leptin influences the physiological effect of compressive forces on cementoblasts, exerting in vitro a proinflammatory and pro-apoptotic effect.

Thu Apr 01 00:00:00 UTC 2021

Response of human cancer cells to simultaneous treatment with sorafenib and radiofrequency current.

Oncology letters

Hernández-Bule ML,Martínez MA,Trillo MÁ,Martínez L,Toledano-Macías E,Úbeda A

44-680G was used in Western Blotting to analyze the effects of simultaneous treatment with sorafenib and 448-kHz, subthermal CRET current on the proliferation and viability of HepG2 cell cultures.

Mon Nov 01 00:00:00 UTC 2021

Response of human cancer cells to simultaneous treatment with sorafenib and radiofrequency current.

Oncology letters

Hernández-Bule ML,Martínez MA,Trillo MÁ,Martínez L,Toledano-Macías E,Úbeda A

44-680G was used in Western Blotting to analyze the effects of simultaneous treatment with sorafenib and 448-kHz, subthermal CRET current on the proliferation and viability of HepG2 cell cultures.

Mon Nov 01 00:00:00 UTC 2021

Hypoxia-inducible factor 1-alpha acts as a bridge factor for crosstalk between ERK1/2 and caspases in hypoxia-induced apoptosis of cementoblasts.

Journal of cellular and molecular medicine

Yong J,von Bremen J,Groeger S,Ruiz-Heiland G,Ruf S

44-680G was used in Western Blotting to provide evidence for the role of ERK1/2 and HIF-1α in the apoptotic response of OCCM-30 cells exposed to CoCl2 -mimicked hypoxia, providing potential new possibilities for molecular intervention in obese patients undergoing orthodontic treatment.

Fri Oct 01 00:00:00 UTC 2021

Adiponectin Interacts In-Vitro With Cementoblasts Influencing Cell Migration, Proliferation and Cementogenesis Partly Through the MAPK Signaling Pathway.

Frontiers in pharmacology

Yong J,von Bremen J,Ruiz-Heiland G,Ruf S

44-680G was used in Western Blotting to conclude that Adiponectin favorably affect OCCM-30 cell migration, proliferation as well as cementogenesis. One of the underlying mechanisms is the activation of MAPK signaling pathway.

Mon Jan 11 00:00:00 UTC 2021

Adiponectin Interacts In-Vitro With Cementoblasts Influencing Cell Migration, Proliferation and Cementogenesis Partly Through the MAPK Signaling Pathway.

Frontiers in pharmacology

Yong J,von Bremen J,Ruiz-Heiland G,Ruf S

Mon Jan 11 00:00:00 UTC 2021

Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages.

Redox biology

Guo C,Davies MJ,Hawkins CL

44-680G was used in Western Blotting to provide further insight into the cellular pathways by which SCN- could exert protective effects on supplementation to decrease the development of chronic inflammatory diseases, such as atherosclerosis.

Tue Sep 01 00:00:00 UTC 2020

A novel protective role of sacubitril/valsartan in cyclophosphamide induced lung injury in rats: impact of miRNA-150-3p on NF-κB/MAPK signaling trajectories.

Scientific reports

Abdel-Latif GA,Elwahab AHA,Hasan RA,ElMongy NF,Ramzy MM,Louka ML,Schaalan MF

44-680G was used in Western Blotting to conclude that sacubitril/valsartan effectively abrogated the CP induced lung inflammation and fibrosis, providing a potential promising protection that could be linked to their ability to inhibit miR-150-3p via inhibition of NF-κB and MAPK signaling pathways.

Mon Aug 03 00:00:00 UTC 2020

A novel protective role of sacubitril/valsartan in cyclophosphamide induced lung injury in rats: impact of miRNA-150-3p on NF-κB/MAPK signaling trajectories.

Scientific reports

Abdel-Latif GA,Elwahab AHA,Hasan RA,ElMongy NF,Ramzy MM,Louka ML,Schaalan MF

Mon Aug 03 00:00:00 UTC 2020

KLF9-dependent ROS regulate melanoma progression in stage-specific manner.

Oncogene

Bagati A,Moparthy S,Fink EE,Bianchi-Smiraglia A,Yun DH,Kolesnikova M,Udartseva OO,Wolff DW,Roll MV,Lipchick BC,Han Z,Kozlova NI,Jowdy P,Berman AE,Box NF,Rodriguez C,Bshara W,Kandel ES,Soengas MS,Paragh G,Nikiforov MA

44-680G was used in Immunohistochemistry to show the ubiquitous transcriptional regulator of oxidative stress KLF9 can mediated the production of ROS in premalignant melanocytes that is required for BRAFV600E-mediated cell proliferation.

Wed May 01 00:00:00 UTC 2019

Simvastatin Mitigates Apoptosis and Transforming Growth Factor-Beta Upregulation in Stretch-Induced Endothelial Cells.

Oxidative medicine and cellular longevity

Dong G,Huang X,Jiang S,Ni L,Chen S

44-680G was used in Western Blotting to determine whether continuous stretch induced by portal hypertension may impair the function of endothelial cells (ECs) in the portal vein and aggravate the progress of portal hypertension and explore its mechanism.

Fri Jun 19 00:00:00 UTC 2020

ANO6 promotes cell proliferation and invasion in glioma through regulating the ERK signaling pathway.

OncoTargets and therapy

Xuan ZB,Wang YJ,Xie J

Published figure using Phospho-ERK1/ERK2 (Thr185, Tyr187) polyclonal antibody (Product # 44-680G) in Western Blot

Mon Apr 11 00:00:00 UTC 2022

Intermittent hypoxia alleviates increased VEGF and pro-angiogenic potential in liver cancer cells.

Oncology letters

Dong G,Lin XH,Liu HH,Gao DM,Cui JF,Ren ZG,Chen RX

44-680G was used in Western Blotting to investigate the ability of intermittent hypoxia to alleviate the acute hypoxia-induced increase of VEGF and decrease the pro-angiogenic potential of liver cancer cells.

Thu Aug 01 00:00:00 UTC 2019

The role of fibroblast growth factor signalling in Echinococcus multilocularis development and host-parasite interaction.

PLoS neglected tropical diseases

Förster S,Koziol U,Schäfer T,Duvoisin R,Cailliau K,Vanderstraete M,Dissous C,Brehm K

44-680G was used in Western Blotting to study the molecular mechanisms of parasite organ tropism towards the liver and the influences of liver cytokines and hormones on parasite development.

Fri Mar 01 00:00:00 UTC 2019

Repeated Sigma-1 Receptor Antagonist MR309 Administration Modulates Central Neuropathic Pain Development After Spinal Cord Injury in Mice.

Frontiers in pharmacology

Castany S,Codony X,Zamanillo D,Merlos M,Verdú E,Boadas-Vaello P

44-680G was used in Western Blotting to evaluate MR309 modulation on both thermal hyperalgesia and mechanical allodynia development after spinal cord injury in mice.

Wed Sep 30 00:00:00 UTC 2020

Response of neuroblastoma cells to RF currents as a function of the signal frequency.

BMC cancer

Hernández-Bule ML,Medel E,Colastra C,Roldán R,Úbeda A

44-680G was used in Immunocytochemistry-immunoflourescence to study the effect of signal frequency in the response of the human neuroblastoma cell line NB69 to subthermal electric treatment.

Thu Sep 05 00:00:00 UTC 2019

Involvement of the EGF Receptor in MAPK Signaling Activation by a 50 Hz Magnetic Field in Human Neuroblastoma Cells.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Martínez MA,Úbeda A,Trillo MÀ

44-680G was used in Immunocytochemistry-immunoflourescence to determine the role of the epidermal growth factor receptor in the field induced cell proliferation and activation of MAPK pathways.

Thu Apr 18 00:00:00 UTC 2019

ZO-1 protein is required for hydrogen peroxide to increase MDCK cell paracellular permeability in an ERK 1/2-dependent manner.

American journal of physiology. Cell physiology

Bilal S,Jaggi S,Janosevic D,Shah N,Teymour S,Voronina A,Watari J,Axis J,Amsler K

44-680G was used in Western Blotting to demonstrate that H2O2 increases MDCK cell paracellular permeability through activation of ERK 1/2.

Sat Sep 01 00:00:00 UTC 2018

FGF21 acts as a negative regulator of bile acid synthesis.

The Journal of endocrinology

Chen MM,Hale C,Stanislaus S,Xu J,Véniant MM

44-680G was used in Western Blotting to determine the role of FGF21 in bile acid metabolism.

Tue May 01 00:00:00 UTC 2018

CRB3 and the FERM protein EPB41L4B regulate proliferation of mammary epithelial cells through the release of amphiregulin.

PloS one

Walker SJ,Selfors LM,Margolis BL,Brugge JS

Published figure using Phospho-ERK1/ERK2 (Thr185, Tyr187) polyclonal antibody (Product # 44-680G) in Western Blot

Tue Apr 16 00:00:00 UTC 2019

Development of MK-8353, an orally administered ERK1/2 inhibitor, in patients with advanced solid tumors.

JCI insight

Moschos SJ,Sullivan RJ,Hwu WJ,Ramanathan RK,Adjei AA,Fong PC,Shapira-Frommer R,Tawbi HA,Rubino J,Rush TS,Zhang D,Miselis NR,Samatar AA,Chun P,Rubin EH,Schiller J,Long BJ,Dayananth P,Carr D,Kirschmeier P,Bishop WR,Deng Y,Cooper A,Shipps GW,Moreno BH,Robert L,Ribas A,Flaherty KT

44-680G was used in Western Blotting to develop an orally bioavailable ERK inhibitor, and demonstrate its efficacy in patients with advanced solid tumours.

Thu Feb 22 00:00:00 UTC 2018

Critical role of sigma-1 receptors in central neuropathic pain-related behaviours after mild spinal cord injury in mice.

Scientific reports

Castany S,Gris G,Vela JM,Verdú E,Boadas-Vaello P

44-680G was used in Western Blotting to show that sigma-1 receptor may modulate central neuropathic pain and point to regulation of sensitisation-related phenomena as a possible mechanism.

Thu Mar 01 00:00:00 UTC 2018

Hematopoietic cell kinase (HCK) is a potential therapeutic target for dysplastic and leukemic cells due to integration of erythropoietin/PI3K pathway and regulation of erythropoiesis: HCK in erythropoietin/PI3K pathway.

Biochimica et biophysica acta. Molecular basis of disease

Roversi FM,Pericole FV,Machado-Neto JA,da Silva Santos Duarte A,Longhini AL,Corrocher FA,Palodetto B,Ferro KP,Rosa RG,Baratti MO,Verjovski-Almeida S,Traina F,Molinari A,Botta M,Saad ST

44680G was used in western blot to characterize a new specific pharmacological hematopoietic cell kinase inhibitor

Wed Feb 01 00:00:00 UTC 2017

WNK1 kinase balances T cell adhesion versus migration in vivo.

Nature immunology

Köchl R,Thelen F,Vanes L,Brazão TF,Fountain K,Xie J,Huang CL,Lyck R,Stein JV,Tybulewicz VL

44680G was used in western blot to discover that WNK1 kinase regulates integrin-mediated adhesion and T cell migration

Thu Sep 01 00:00:00 UTC 2016

ERK and p38 MAPK Activities Determine Sensitivity to PI3K/mTOR Inhibition via Regulation of MYC and YAP.

Cancer research

Muranen T,Selfors LM,Hwang J,Gallegos LL,Coloff JL,Thoreen CC,Kang SA,Sabatini DM,Mills GB,Brugge JS

Published figure using Phospho-ERK1/ERK2 (Thr185, Tyr187) polyclonal antibody (Product # 44-680G) in Western Blot

Thu Dec 15 00:00:00 UTC 2016

Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell death.

Oncogene

Salas E,Roy S,Marsh T,Rubin B,Debnath J

44-680G was used in western blot to show that inhibition of the oxidative arm of the pentose phosphate pathway is required for antimalarial-mediated apoptosis.

Thu Jun 02 00:00:00 UTC 2016

Targeting the PI3K/AKT/mTOR pathway overcomes the stimulating effect of dabrafenib on the invasive behavior of melanoma cells with acquired resistance to the BRAF inhibitor.

International journal of oncology

Caporali S,Alvino E,Lacal PM,Levati L,Giurato G,Memoli D,Caprini E,Antonini Cappellini GC,D'Atri S

44680G was used in western blot to compare dabrafenib-resistant and -sensitive BRAF-mutant human melanoma cell lines

Thu Sep 01 00:00:00 UTC 2016

Endosomal Na+/H+ exchanger NHE5 influences MET recycling and cell migration.

Molecular biology of the cell

Fan SH,Numata Y,Numata M

44-680G was used in western blot to investigate the role of neuron-enriched Na(+)/H(+) exchanger NHE5

Mon Feb 15 00:00:00 UTC 2016

Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance.

Nature communications

Kabe Y,Nakane T,Koike I,Yamamoto T,Sugiura Y,Harada E,Sugase K,Shimamura T,Ohmura M,Muraoka K,Yamamoto A,Uchida T,Iwata S,Yamaguchi Y,Krayukhina E,Noda M,Handa H,Ishimori K,Uchiyama S,Kobayashi T,Suematsu M

44-680G was used in western blot to determine facilitation of chemoresistance and cancer proliferation by Haem-dependent dimerization of PGRMC1/Sigma-2 receptor

Fri Mar 18 00:00:00 UTC 2016

Anti-c-Met monoclonal antibody ABT-700 breaks oncogene addiction in tumors with MET amplification.

BMC cancer

Wang J,Goetsch L,Tucker L,Zhang Q,Gonzalez A,Vaidya KS,Oleksijew A,Boghaert E,Song M,Sokolova I,Pestova E,Anderson M,Pappano WN,Ansell P,Bhathena A,Naumovski L,Corvaia N,Reilly EB

44-680G was used in immunohistochemistry - paraffin section and western blot to learn how oncogene addiction can be broken in tumors with MET amplification by the Anti-c-Met monoclonal antibody ABT-700

Tue Feb 16 00:00:00 UTC 2016

Anti-c-Met monoclonal antibody ABT-700 breaks oncogene addiction in tumors with MET amplification.

BMC cancer

Wang J,Goetsch L,Tucker L,Zhang Q,Gonzalez A,Vaidya KS,Oleksijew A,Boghaert E,Song M,Sokolova I,Pestova E,Anderson M,Pappano WN,Ansell P,Bhathena A,Naumovski L,Corvaia N,Reilly EB

Tue Feb 16 00:00:00 UTC 2016

Power Frequency Magnetic Fields Affect the p38 MAPK-Mediated Regulation of NB69 Cell Proliferation Implication of Free Radicals.

International journal of molecular sciences

Martínez MA,Úbeda A,Moreno J,Trillo MÁ

44-680G was used in Immunocytochemistry to investigate the effect of magnetic fields on the p38 MAPK-mediated regulation of NB69 cell proliferation and the potential involvement of free radicals.

Wed Apr 06 00:00:00 UTC 2016

ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.

Nature cell biology

Murrow L,Malhotra R,Debnath J

44-680G was used in western blot to uncover a role for ATG12-ATG3 in late endosome function that is distinct from autophagy.

Sun Mar 01 00:00:00 UTC 2015

A new water soluble MAPK activator exerts antitumor activity in melanoma cells resistant to the BRAF inhibitor vemurafenib.

Biochemical pharmacology

Graziani G,Artuso S,De Luca A,Muzi A,Rotili D,Scimeca M,Atzori MG,Ceci C,Mai A,Leonetti C,Levati L,Bonanno E,Tentori L,Caccuri AM

44-680G was used in western blot to test if NBDHEX and MC3181 have antitumor activity against melanoma cells resistant to vemurafenib

Fri May 01 00:00:00 UTC 2015

All-Trans Retinoic Acid Induces Proliferation, Survival, and Migration in A549 Lung Cancer Cells by Activating the ERK Signaling Pathway through a Transcription-Independent Mechanism.

BioMed research international

Quintero Barceinas RS,García-Regalado A,Aréchaga-Ocampo E,Villegas-Sepúlveda N,González-De la Rosa CH

44-680G was used in western blot to study how all-trans retinoic acid modulates the ERK signaling pathway

Fri Aug 26 00:00:00 UTC 2016

All-Trans Retinoic Acid Induces Proliferation, Survival, and Migration in A549 Lung Cancer Cells by Activating the ERK Signaling Pathway through a Transcription-Independent Mechanism.

BioMed research international

Quintero Barceinas RS,García-Regalado A,Aréchaga-Ocampo E,Villegas-Sepúlveda N,González-De la Rosa CH

44-680G was used in western blot to study how all-trans retinoic acid modulates the ERK signaling pathway

Fri Aug 26 00:00:00 UTC 2016

A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis.

PloS one

Cheng Z,Liu F,Zhu S,Tian H,Wang L,Wang Y

44-680G was used in immunocytochemistry to describe a method for the preparation, fixation, and fluorescence analysis of in vitro cultivated metacestode vesicles from E. multilocularis

Fri Jan 22 00:00:00 UTC 2016

Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells.

International journal of molecular sciences

Shih MF,Pan KH,Cherng JY

44-680G was used in western blot to test if DEHP affects MMP-2 or MMP-9 expression in vascular smooth muscle cells

Fri Dec 04 00:00:00 UTC 2015

Candidate tumor suppressor B-cell translocation gene 3 impedes neoplastic progression by suppression of AKT.

Cell death & disease

Cheng YC,Chen PH,Chiang HY,Suen CS,Hwang MJ,Lin TY,Yang HC,Lin WC,Lai PL,Shieh SY

44-680G was used in western blot to elucidate the tumor suppressor function of BTG3

Thu Jan 08 00:00:00 UTC 2015

Increased ERK and JNK activation and decreased ERK/JNK ratio are associated with long-term organ damage in patients with systemic lupus erythematosus.

Rheumatology (Oxford, England)

Bloch O,Amit-Vazina M,Yona E,Molad Y,Rapoport MJ

44-680G was used in western blot to test if extracellular signal-regulated kinase and c-Jun N-terminal kinase are associated with long-term organ damage in SLE patients.

Sun Jun 01 00:00:00 UTC 2014

The MEK1/2-ERK1/2 pathway is activated in chronic rhinosinusitis with nasal polyps.

Archivum immunologiae et therapiae experimentalis

Linke R,Pries R,Könnecke M,Bruchhage KL,Böscke R,Gebhard M,Wollenberg B

44-680G was used in immunohistochemistry and western blot to assess the role of the MEK/2-ERK/2 signaling pathway in the pathogenesis of chronic rhinosinusitis with nasal polyps.

Sun Jun 01 00:00:00 UTC 2014

Silver and fullerene nanoparticles' effect on interleukin-2-dependent proliferation of CD4 (+) T cells.

Toxicology in vitro : an international journal published in association with BIBRA

Côté-Maurais G,Bernier J

44-680G was used in western blot to determine if nanoparticles inhibit IL-2-dependent T cell proliferation.

Mon Dec 01 00:00:00 UTC 2014

The C-terminus of human nucleotide receptor P2X7 is critical for receptor oligomerization and N-linked glycosylation.

PloS one

Wickert LE,Blanchette JB,Waldschmidt NV,Bertics PJ,Denu JM,Denlinger LC,Lenertz LY

44-680G was used in western blot to alter the C-terminal trafficking domain of P2X7 and examine its function.

Mon Dec 30 00:00:00 UTC 2013

Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling.

BMC immunology

Smeets RL,Fleuren WW,He X,Vink PM,Wijnands F,Gorecka M,Klop H,Bauerschmidt S,Garritsen A,Koenen HJ,Joosten I,Boots AM,Alkema W

Wed Mar 14 00:00:00 UTC 2012

TFG-1 function in protein secretion and oncogenesis.

Nature cell biology

Witte K,Schuh AL,Hegermann J,Sarkeshik A,Mayers JR,Schwarze K,Yates JR,Eimer S,Audhya A

44-680G was used in immunocytochemistry to identify and characterize the role of TFG-1 in protein secretion and export of cargo from the endoplasmic reticulum.

Sun May 01 00:00:00 UTC 2011

TFG-1 function in protein secretion and oncogenesis.

Nature cell biology

Witte K,Schuh AL,Hegermann J,Sarkeshik A,Mayers JR,Schwarze K,Yates JR,Eimer S,Audhya A

44-680G was used in immunocytochemistry to identify and characterize the role of TFG-1 in protein secretion and export of cargo from the endoplasmic reticulum.

Sun May 01 00:00:00 UTC 2011

ErbB2 stabilizes epidermal growth factor receptor (EGFR) expression via Erk and Sprouty2 in extracellular matrix-detached cells.

The Journal of biological chemistry

Grassian AR,Schafer ZT,Brugge JS

44-680G was used in western blot to investigate the roles of EGFR and beta1 integrin in ErbB2-driven anchorage independence of tumor cells

Fri Jan 07 00:00:00 UTC 2011

ErbB2 stabilizes epidermal growth factor receptor (EGFR) expression via Erk and Sprouty2 in extracellular matrix-detached cells.

The Journal of biological chemistry

Grassian AR,Schafer ZT,Brugge JS

44-680G was used in western blot to investigate the roles of EGFR and beta1 integrin in ErbB2-driven anchorage independence of tumor cells

Fri Jan 07 00:00:00 UTC 2011

3-phosphoinositide-dependent protein kinase-1 regulates proliferation and survival of cancer cells with an activated mitogen-activated protein kinase pathway.

Molecular cancer research : MCR

Lu Z,Cox-Hipkin MA,Windsor WT,Boyapati A

44-680G was used in western blot to examine how PDK1 affects MAPK and PI3K signaling in tumor cells

Mon Mar 01 00:00:00 UTC 2010

Mutation of putative N-linked glycosylation sites on the human nucleotide receptor P2X7 reveals a key residue important for receptor function.

Biochemistry

Lenertz LY,Wang Z,Guadarrama A,Hill LM,Gavala ML,Bertics PJ

Published figure using Phospho-ERK1/ERK2 (Thr185, Tyr187) polyclonal antibody (Product # 44-680G) in Western Blot

Tue Jun 08 00:00:00 UTC 2010

Induction of invasion in an organotypic oral cancer model by CoCl2, a hypoxia mimetic.

European journal of oral sciences

Brusevold IJ,Husvik C,Schreurs O,Schenck K,Bryne M,Søland TM

44-680G was used in immunohistochemistry to develop an in vitro model for experimental studies of cancer cell invasion

Thu Apr 01 00:00:00 UTC 2010

Extranuclear estrogen receptors mediate the neuroprotective effects of estrogen in the rat hippocampus.

PloS one

Yang LC,Zhang QG,Zhou CF,Yang F,Zhang YD,Wang RM,Brann DW

44-680G was used in immunocytochemistry and western blot to investigate the potential neuroprotective effects of extranuclear estrogen receptors in the brain following cerebral ischemia

Fri May 07 00:00:00 UTC 2010

Extranuclear estrogen receptors mediate the neuroprotective effects of estrogen in the rat hippocampus.

PloS one

Yang LC,Zhang QG,Zhou CF,Yang F,Zhang YD,Wang RM,Brann DW

44-680G was used in immunocytochemistry and western blot to investigate the potential neuroprotective effects of extranuclear estrogen receptors in the brain following cerebral ischemia

Fri May 07 00:00:00 UTC 2010

Extranuclear estrogen receptors mediate the neuroprotective effects of estrogen in the rat hippocampus.

PloS one

Yang LC,Zhang QG,Zhou CF,Yang F,Zhang YD,Wang RM,Brann DW

44-680G was used in immunocytochemistry and western blot to investigate the potential neuroprotective effects of extranuclear estrogen receptors in the brain following cerebral ischemia

Fri May 07 00:00:00 UTC 2010

Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment.

Nature

Schafer ZT,Grassian AR,Song L,Jiang Z,Gerhart-Hines Z,Irie HY,Gao S,Puigserver P,Brugge JS

Published figure using Phospho-ERK1/ERK2 (Thr185, Tyr187) polyclonal antibody (Product # 44-680G) in Western Blot

Thu Sep 03 00:00:00 UTC 2009

The absence of caveolin-1 increases proliferation and anchorage- independent growth by a Rac-dependent, Erk-independent mechanism.

Molecular and cellular biology

Cerezo A,Guadamillas MC,Goetz JG,Sánchez-Perales S,Klein E,Assoian RK,del Pozo MA

44-680G was used in western blot to elucidate the mechanisms by which loss of Cav1 function promotes cancer cell growth

Tue Sep 01 00:00:00 UTC 2009

Dissection of the osteogenic effects of laminin-332 utilizing specific LG domains: LG3 induces osteogenic differentiation, but not mineralization.

Experimental cell research

Klees RF,Salasznyk RM,Ward DF,Crone DE,Williams WA,Harris MP,Boskey A,Quaranta V,Plopper GE

44-680G was used in western blot to study the role of laminins during osteogenic differentiation of human mesenchymal stem cells

Fri Feb 15 00:00:00 UTC 2008

A study of phosphorylated ERK1/2 and COX-2 in early stage (T1-T2) oral squamous cell carcinomas.

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

Søland TM,Husvik C,Koppang HS,Boysen M,Sandvik L,Clausen OP,Christoffersen T,Bryne M

44-680G was used in immunohistochemistry - paraffin section to assess the prognostic value of activated phosphorylated ERK1 and ERK2 and COX-2 at the invasive front and in central/superficial parts of oral squamous cell carcinomas

Wed Oct 01 00:00:00 UTC 2008

Quantitative measurement of epidermal growth factor receptor-mitogen-activated protein kinase signal transduction using a nine-plex, peptide-based immunoassay.

Analytical biochemistry

Rauh-Adelmann C,Moskow JM,Graham JR,Yen LG,Boucher JI,Murphy CE,Nadler TK,Gordon NF,Radding JA

44-680G was used in western blot to develop an assay to measure temporal, site-specific phosphorylation of key members of the EGFR pathway in A431 cells stimulated with epidermal growth factor

Tue Apr 15 00:00:00 UTC 2008

Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.

Journal of leukocyte biology

Ratthé C,Pelletier M,Chiasson S,Girard D

Tue May 01 00:00:00 UTC 2007

Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5.

Journal of cellular biochemistry

Salasznyk RM,Klees RF,Boskey A,Plopper GE

44-680G was used in western blot to report that FAK is an important mediator of laminin-5-induced osteogenic differentiation of human mesenchymal stem cells

Thu Feb 01 00:00:00 UTC 2007

The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.

Gynecologic oncology

Davidson B,Konstantinovsky S,Kleinberg L,Nguyen MT,Bassarova A,Kvalheim G,Nesland JM,Reich R

Fri Sep 01 00:00:00 UTC 2006

Epidermal growth factor receptor induced Erk phosphorylation in the suprachiasmatic nucleus.

Brain research

Hao H,Schwaber J

44-680G was used in immunohistochemistry - free floating to report that epidermal growth factor treatment induced Erk1/2 phosphorylation in rat suprachiasmatic nucleus cells

Tue May 09 00:00:00 UTC 2006

Phospho-caveolin-1 mediates integrin-regulated membrane domain internalization.

Nature cell biology

del Pozo MA,Balasubramanian N,Alderson NB,Kiosses WB,Grande-García A,Anderson RG,Schwartz MA

Thu Sep 01 00:00:00 UTC 2005

Phospho-caveolin-1 mediates integrin-regulated membrane domain internalization.

Nature cell biology

del Pozo MA,Balasubramanian N,Alderson NB,Kiosses WB,Grande-García A,Anderson RG,Schwartz MA

Thu Sep 01 00:00:00 UTC 2005

CHIR-258: a potent inhibitor of FLT3 kinase in experimental tumor xenograft models of human acute myelogenous leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research

Lopes de Menezes DE,Peng J,Garrett EN,Louie SG,Lee SH,Wiesmann M,Tang Y,Shephard L,Goldbeck C,Oei Y,Ye H,Aukerman SL,Heise C

Fri Jul 15 00:00:00 UTC 2005

Mitogen-activated protein kinase expression and activation does not differentiate benign from malignant mesothelial cells.

Cancer

Vintman L,Nielsen S,Berner A,Reich R,Davidson B

Wed Jun 01 00:00:00 UTC 2005

Heat shock protein 60 inhibits Th1-mediated hepatitis model via innate regulation of Th1/Th2 transcription factors and cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Zanin-Zhorov A,Bruck R,Tal G,Oren S,Aeed H,Hershkoviz R,Cohen IR,Lider O

Tue Mar 15 00:00:00 UTC 2005

Heat shock protein 60 inhibits Th1-mediated hepatitis model via innate regulation of Th1/Th2 transcription factors and cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Zanin-Zhorov A,Bruck R,Tal G,Oren S,Aeed H,Hershkoviz R,Cohen IR,Lider O

Tue Mar 15 00:00:00 UTC 2005

Chemokine receptor CCR2 expression by systemic sclerosis fibroblasts: evidence for autocrine regulation of myofibroblast differentiation.

Arthritis and rheumatism

Carulli MT,Ong VH,Ponticos M,Shiwen X,Abraham DJ,Black CM,Denton CP

44-680G was used in western blot to assess the contribution of CCL2/CCR2 signaling to systemic sclerosis

Thu Dec 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-1; ERK-2; ERK/rolled; erk1 erk2; ERK1b; ERT1; ERT2; extracellular signal-regulated kinase; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; Insulin-stimulated MAP2 kinase; M phase MAP kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAP-kinase; MAPK 1; MAPK 2; MAPK 3; MBP kinase; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 1a; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; Myelin basic protein kinase; Myelin xP42 protein kinase; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; phospho-ERK; pp42/MAP kinase; Protein rolled; protein tyrosine kinase ERK2; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; rolled; sevenmaker

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Gene Aliases: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; BOS_16809; BOS_22892; C78273; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmErk; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; GroupII; HS44KDAP; HUMKER1A; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; mapk1-a; mapk1-b; mapk1a; MAPK2; MAPK3; MNK1; mpk1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B; XELAEV_18010534mg; xp42

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UniProt ID: (Bovine) P46196, (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844, (Xenopus) Q5D061, (Fruit fly) P40417

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Entrez Gene ID: (Bovine) 327672, (Chicken) 373953, (Human) 5594, (Bovine) 531391, (Human) 5595, (Mouse) 26413, (Rat) 116590, (Rat) 50689, (Mouse) 26417, (Xenopus) 398985, (Fruit fly) 3354888

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Function(s)

phosphotyrosine binding protein serine/threonine kinase activity MAP kinase activity ATP binding RNA polymerase II carboxy-terminal domain kinase activity phosphatase binding DNA binding protein binding transcription factor binding kinase activity mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding JUN kinase activity

Process(es)

Performance Guarantee

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Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal Antibody

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (44-680G) in IHC (P)

Product Details

44-680G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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