Invitrogen

Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (700012) in ICC/IF

Immunofluorescence analysis of Phosph-ERK1/2 (Thr 185,Tyr187) was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK2 Rabbit monoclonal Antibody (Product # 700012) at 5 µg/mL in 0.1% BSA and incubated over... View More

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunohistochemistry (IHC)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody

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Product Details

700012

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

ELISA (ELISA)

Assay-dependent

ChIP assay (ChIP)

1 µL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

15H10L7

Immunogen

A peptide corresponding to amino acids 198-208 and 181-191 of P27361 and P28482, respectively.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532267

Product Specific Information

700012 has successfully been used in western blot to detect phospho-ERK1/2 in TPA-treated HeLa, NRK and MDCK cell lysates.

This antibody is predicted to react with bovine, chicken, chimpanzee, mouse, rat, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dasabuvir suppresses esophageal squamous cell carcinoma growth in vitro and in vivo through targeting ROCK1.

Cell death & disease

Liu X,Jiang Y,Zhou H,Zhao X,Li M,Bao Z,Wang Z,Zhang C,Xie Z,Zhao J,Dong Z,Liu K,Guo Z

700012 was used in ChIP Assay, ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to provide evidence that dasabuvir suppresses ESCC growth in vivo and in vitro through blocking ROCK1/ERK signaling pathway.

Mon Feb 13 00:00:00 UTC 2023

Temporal activation of WNT/β-catenin signaling is sufficient to inhibit SOX10 expression and block melanoma growth.

Oncogene

Uka R,Britschgi C,Krättli A,Matter C,Mihic D,Okoniewski MJ,Gualandi M,Stupp R,Cinelli P,Dummer R,Levesque MP,Shakhova O

700012 was used in Immunohistochemistry-immunofluorescence to show that MEK and RAF inhibitors do not suppress levels of SOX10 protein in patient-derived cells in vitro, as well as in melanoma patients in vivo.

Fri May 01 00:00:00 UTC 2020

Temporal activation of WNT/β-catenin signaling is sufficient to inhibit SOX10 expression and block melanoma growth.

Oncogene

Uka R,Britschgi C,Krättli A,Matter C,Mihic D,Okoniewski MJ,Gualandi M,Stupp R,Cinelli P,Dummer R,Levesque MP,Shakhova O

Fri May 01 00:00:00 UTC 2020

HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer.

Molecular pharmaceutics

Pereira PMR,Ragupathi A,Shmuel S,Mandleywala K,Viola NT,Lewis JS

700012 was used in Western Blotting to investigate whether degradation of hyaluronan can resensitize HER2-overexpressing breast cancer cells to trastuzumab.

Mon Jan 06 00:00:00 UTC 2020

LQB‑118 compound inhibits migration and induces cell death in glioblastoma cells.

Oncology reports

Bernardo PS,Guimarães GHC,De Faria FCC,Longo GMDC,Lopes GPF,Netto CD,Costa PRR,Maia RC

700012 was used in Western Blotting to demonstrate that LQB‚118 maintained its effectiveness in a 3D cell conformation, which shares more similarities with the tumor mass.

Wed Jan 01 00:00:00 UTC 2020

Alterations of the renin angiotensin system in human end-stage heart failure before and after mechanical cardiac unloading by LVAD support.

Molecular and cellular biochemistry

Messmann R,Dietl A,Wagner S,Domenig O,Jungbauer C,Luchner A,Maier LS,Schopka S,Hirt S,Schmid C,Birner C

700012 was used in Western Blotting to determine the regulation patterns of key components of the RAS and the β-arrestin signaling pathways in left ventricular (LV) tissue.

Tue Sep 01 00:00:00 UTC 2020

Disc1 Carrier Mice Exhibit Alterations in Neural pIGF-1Rβ and Related Kinase Expression.

Frontiers in cellular neuroscience

Sultana R,Shrestha A,Lee CC,Ogundele OM

700012 was used in Western Blotting to demonstrate that the expression of active IGF-1Rβ (pIGF-1Rβ) is altered in the hippocampus and prefrontal cortex (PFC) of disc1 mutant mice and vary with the dose of the mutation (homozygous and heterozygous).

Mon Sep 28 00:00:00 UTC 2020

Disc1 Carrier Mice Exhibit Alterations in Neural pIGF-1Rβ and Related Kinase Expression.

Frontiers in cellular neuroscience

Sultana R,Shrestha A,Lee CC,Ogundele OM

Mon Sep 28 00:00:00 UTC 2020

SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.

Frontiers in synaptic neuroscience

Shrestha A,Sultana R,Lee CC,Ogundele OM

700012 was used in Western Blotting to demonstrate that positive modulation of SK channel at hippocampal synapses likely refine GluN1-linked plasticity by tuning dendritic localization of CaMKIIα.

Sun Apr 21 00:00:00 UTC 2024

Neonatal maternal deprivation impairs localized de novo activity-induced protein translation at the synapse in the rat hippocampus.

Bioscience reports

Ahmad F,Salahuddin M,Alsamman K,Herzallah HK,Al-Otaibi ST

700012 was used in Western Blotting to elucidate the molecular mechanisms underlying the detrimental alterations in brain functions induced by early-life stress.

Fri Jun 29 00:00:00 UTC 2018

Metformin exerts multitarget antileukemia activity in JAK2^V617F-positive myeloproliferative neoplasms.

Cell death & disease

Machado-Neto JA,Fenerich BA,Scopim-Ribeiro R,Eide CA,Coelho-Silva JL,Dechandt CRP,Fernandes JC,Rodrigues Alves APN,Scheucher PS,Simões BP,Alberici LC,de Figueiredo Pontes LL,Tognon CE,Druker BJ,Rego EM,Traina F

Published figure using Phospho-ERK1/ERK2 (Thr185, Tyr187) recombinant monoclonal antibody (Product # 700012) in Western Blot

Thu Feb 22 00:00:00 UTC 2018

Integrator orchestrates RAS/ERK1/2 signaling transcriptional programs.

Genes & development

Yue J,Lai F,Beckedorff F,Zhang A,Pastori C,Shiekhattar R

700012 was used in Chromatin immunoprecipitation to demonstrate that the MAPK transcriptional response in human cells is funneled through Integrator, an RNA polymerase II-associated complex.

Fri Sep 01 00:00:00 UTC 2017

ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells.

Biochimica et biophysica acta

Machado-Neto JA,Lazarini M,Favaro P,de Melo Campos P,Scopim-Ribeiro R,Franchi Junior GC,Nowill AE,Lima PR,Costa FF,Benichou S,Olalla Saad ST,Traina F

700012 was used in western blot to identify binding partners ANKHD1 in leukemia cells

Sun Mar 01 00:00:00 UTC 2015

TGF-β mediates early angiogenesis and latent fibrosis in an Emilin1-deficient mouse model of aortic valve disease.

Disease models & mechanisms

Munjal C,Opoka AM,Osinska H,James JF,Bressan GM,Hinton RB

700012 was used in Immunohistochemistry to show that manifestation of EFF and aberrant angiogenesis suggests they are crucial intermediate factors involved in aortic valve disease.

Fri Aug 01 00:00:00 UTC 2014

Multidimensional profiling of CSF1R screening hits and inhibitors: assessing cellular activity, target residence time, and selectivity in a higher throughput way.

Journal of biomolecular screening

Uitdehaag JC,Sünnen CM,van Doornmalen AM,de Rouw N,Oubrie A,Azevedo R,Ziebell M,Nickbarg E,Karstens WJ,Ruygrok S

700012 was used in western blot to develop medium throughput assays to characterize CSF1R HTS hits and aid the selection of the best compounds for lead progression

Sat Oct 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44-ERK1; p44-MAPK; protein tyrosine kinase ERK2

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Gene Aliases: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Rat) P21708

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Entrez Gene ID: (Human) 5594, (Dog) 477575, (Human) 5595, (Dog) 100855877, (Rat) 116590, (Rat) 50689

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Function(s)

phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding protein kinase activity protein kinase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (700012) in ICC/IF

Product Details

700012

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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