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Phospho-EphA8 (Tyr839) Antibody (PA5-105876) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-EphA8 (Tyr838) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-EphA8 (Tyr838) polyclonal antibody (Product # PA5-105876) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105876
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EPHA8(Accession P29322), corresponding to amino acid residues around phosphorylated Tyr839.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817275
Product Specific Information
Antibody detects endogenous levels of EPHA8 only when phosphorylated at Tyr839.
Target Information
EPHA8 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. EPHA8 plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EK3; epa8; EPH- and ELK-related kinase; EPH- and ELK-related tyrosine kinase; EPH-like kinase 3; Ephrin type-A receptor 8; Ephrin type-A receptor 8 precursor (Tyrosine-protein kinase receptor EEK) (EPH-and ELK-related kinase); hydroxyaryl-protein kinase; protein-tyrosine kinase; Tyrosine-protein kinase receptor EEK; tyrosylprotein kinase
Gene Aliases: AW047546; EEK; EK3; EPHA8; HEK3; KIAA1459; mKIAA1459
UniProt ID: (Human) P29322, (Mouse) O09127
Entrez Gene ID: (Human) 2046, (Rat) 60589, (Mouse) 13842
GPI-linked ephrin receptor activity
ATP binding
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
substrate-dependent cell migration
cell adhesion
axon guidance
neuron remodeling
peptidyl-tyrosine phosphorylation
regulation of cell adhesion
neuron projection development
regulation of cell adhesion mediated by integrin
positive regulation of MAPK cascade
positive regulation of phosphatidylinositol 3-kinase activity
protein autophosphorylation
ephrin receptor signaling pathway
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
nervous system development
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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