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Phospho-EphB6 (Tyr645) Antibody (PA5-106136) in ICC/IF
Immunofluorescent analysis of Phospho-EphB6 (Tyr630) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-EphB6 (Tyr630) polyclonal antibody (Product # PA5-106136) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 59... View More
Product Details
PA5-106136
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EPHB6(Accession O15197), corresponding to amino acid residues around phosphorylated Tyr645.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2817534
Product Specific Information
Antibody detects endogenous levels of EPHB6 only when phosphorylated at Tyr645.
Target Information
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ephrin receptor encoded by this gene lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chicken eph/elk receptor-like protein; EPB6; EphB6v; Epherin B6; Epherin receptor B6; ephrin type A receptor; Ephrin type-B receptor 6; HEP; human kinase-defective Eph-family receptor protein; MEP; MGC129910; MGC129911; Tyrosine-protein kinase-defective receptor EPH-6
Gene Aliases: Cekl; EPHB6; HEP; Mep
UniProt ID: (Human) O15197, (Rat) P0C0K7, (Mouse) O08644
Entrez Gene ID: (Human) 2051, (Rat) 312275, (Mouse) 13848
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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