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Invitrogen
Phospho-Ephrin B1/B2/B3 (Tyr324) Polyclonal Antibody
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Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody (PA5-103267) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-Ephrin B1/B2/B3 (Tyr324) in human gastric tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Ephrin B1/B2/B3 (Tyr324) polyclonal antibody (Product # PA5-103267) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-103267
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EFNB1(Accession P98172), corresponding to amino acid residues around phosphorylated Tyr324.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815966
Product Specific Information
Antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324.
Target Information
Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CEK5 receptor ligand; CEK5-L; EFL-3; ELF-2; ELK ligand; ELK-L; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 8; ephrin ligand B3; Ephrin-B1; Ephrin-B2; Ephrin-B3; HTK ligand; HTK-L; LERK-2; LERK-5; LERK-8; ligand of eph-related kinase 5; Stimulated by retinoic acid gene 1 protein
Gene Aliases: Cek5-L; CFND; CFNS; EFB1; EFL-3; EFL-6; EFL3; EFL6; EFNB1; EFNB2; EFNB3; ELF-2; ELF-3; Elf2; Elk-L; ELK-L3; Epl2; Epl5; Epl8; EPLG2; EPLG5; EPLG8; Htk-L; HTKL; LERK-2; LERK-5; LERK-8; LERK2; LERK5; LERK8; NLERK-1; NLERK-2; Stra1
UniProt ID: (Human) P98172, (Human) P52799, (Human) Q15768, (Mouse) P52795, (Mouse) P52800, (Mouse) O35393
Entrez Gene ID: (Human) 1947, (Human) 1948, (Human) 1949, (Rat) 25186, (Mouse) 13641, (Mouse) 13642, (Rat) 306636, (Mouse) 13643, (Rat) 360546
angiogenesis
lymph vessel development
cell migration involved in sprouting angiogenesis
cell adhesion
multicellular organism development
nervous system development
axon guidance
organ morphogenesis
negative regulation of keratinocyte proliferation
cell migration
cell differentiation
ephrin receptor signaling pathway
blood vessel morphogenesis
regulation of chemotaxis
nephric duct morphogenesis
positive regulation of cardiac muscle cell differentiation
neural crest cell migration
embryonic pattern specification
positive regulation of T cell proliferation
adult walking behavior
axon choice point recognition
cell-cell signaling
T cell costimulation
viral entry into host cell
anatomical structure morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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