Invitrogen

Phospho-FAK (Tyr397) Monoclonal Antibody (141-9)

44 Published Figures

62 References

View all (101) FAK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (44)

Phospho-FAK (Tyr397) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 48

Phospho-FAK (Tyr397) Antibody (44-625G) in ICC/IF

HeLa cells were fixed prior to immunostaining with the Rabbit anti-FAK (pY397) monoclonal antibody. The signal was detected with an anti-rabbit FITC conjugated secondary antibody. The data shows that the antibody detected phosphorylated FAK localized at focal adhesions. Cells were counterstained with DAPI. (Product # 44-625G)

Phospho-FAK (Tyr397) Antibody in Western Blot (WB)

Phospho-FAK (Tyr397) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Phospho-FAK (Tyr397) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-FAK (Tyr397) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Phospho-FAK (Tyr397) Antibody in Immunohistochemistry (IHC)

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Product Details

44-625G

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 39 publications 39 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 10 publications 10 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Fruit fly, Human, Mouse, Rat, Xenopus

Published species

Fish, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

141-9

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533702

Product Specific Information

This antibody will cross-react with the corresponding autophosphorylation site on Proline-rich/Ca2+- activated tyrosine kinase (Pyk2), [pY402]. FAK [pY397] polyclonal antibody (Cat. No. 44-624G) does not cross-react with Pyk2.

The antibody has been used in western blotting. Previous lots of this antibody have been used in immunocytochemistry. For western blotting applications, we recommend using the antibody at a 1:1,000 starting dilution. For immunofluorecence staining applications, we recommend a 1:50 starting dilution. Positive control used: Primary chicken embryo fibroblasts expressing human FAK and plated on fibronectin.

We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20° C. For shipment or short-term storage (up to one week), 2-8° C is sufficient. Expires one year from date of receipt when stored as instructed.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SMAD2/3 mediate oncogenic effects of TGF-β in the absence of SMAD4.

Communications biology

Bertrand-Chapel A,Caligaris C,Fenouil T,Savary C,Aires S,Martel S,Huchedé P,Chassot C,Chauvet V,Cardot-Ruffino V,Morel AP,Subtil F,Mohkam K,Mabrut JY,Tonon L,Viari A,Cassier P,Hervieu V,Castets M,Mauviel A,Sentis S,Bartholin L

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Fri Oct 07 00:00:00 UTC 2022

Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFβ1.

Molecular biology of the cell

Kilinc AN,Han S,Barrett LA,Anandasivam N,Nelson CM

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Immunohistochemistry

Mon Mar 01 00:00:00 UTC 2021

Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFβ1.

Molecular biology of the cell

Kilinc AN,Han S,Barrett LA,Anandasivam N,Nelson CM

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Immunohistochemistry

Mon Mar 01 00:00:00 UTC 2021

Intravital imaging technology guides FAK-mediated priming in pancreatic cancer precision medicine according to Merlin status.

Science advances

Murphy KJ,Reed DA,Vennin C,Conway JRW,Nobis M,Yin JX,Chambers CR,Pereira BA,Lee V,Filipe EC,Trpceski M,Ritchie S,Lucas MC,Warren SC,Skhinas JN,Magenau A,Metcalf XL,Stoehr J,Major G,Parkin A,Bidanel R,Lyons RJ,Zaratzian A,Tayao M,Da Silva A,Abdulkhalek L,Gill AJ,Johns AL,Biankin AV,Samra J,Grimmond SM,Chou A,Goetz JG,Samuel MS,Lyons JG,Burgess A,Caldon CE,Horvath LG,Daly RJ,Gadegaard N,Wang Y,Sansom OJ,Morton JP,Cox TR,Pajic M,Herrmann D,Timpson P

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Fri Oct 01 00:00:00 UTC 2021

Inhibiting FAK-Paxillin Interaction Reduces Migration and Invadopodia-Mediated Matrix Degradation in Metastatic Melanoma Cells.

Cancers

Mousson A,Legrand M,Steffan T,Vauchelles R,Carl P,Gies JP,Lehmann M,Zuber G,De Mey J,Dujardin D,Sick E,Rondé P

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Wed Apr 14 00:00:00 UTC 2021

Cell adhesion is regulated by CDK1 during the cell cycle.

The Journal of cell biology

Jones MC,Askari JA,Humphries JD,Humphries MJ

44-625G was used in Western Blotting to demonstrate that cyclin-dependent kinase 1 mediates the link between the cell cycle machinery and the regulation of cell adhesion to the extracellular matrix.

Mon Sep 03 00:00:00 UTC 2018

Targeting CXCR4 and FAK reverses doxorubicin resistance and suppresses invasion in non-small cell lung carcinoma.

Cellular oncology (Dordrecht, Netherlands)

Dragoj M,Milosevic Z,Bankovic J,Tanic N,Pesic M,Stankovic T

44-625G was used in western blot to study pathways involved in metastases and therapeutic resistance of non-small cell lung carcinoma

Wed Feb 01 00:00:00 UTC 2017

Neural-specific deletion of the focal adhesion adaptor protein paxillin slows migration speed and delays cortical layer formation.

Development (Cambridge, England)

Rashid M,Belmont J,Carpenter D,Turner CE,Olson EC

44-625G was used in Western Blotting to study the roles of paxillin and Hic-5 in cortical development.

Wed Nov 01 00:00:00 UTC 2017

Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix.

Molecular biology of the cell

Mekhdjian AH,Kai F,Rubashkin MG,Prahl LS,Przybyla LM,McGregor AL,Bell ES,Barnes JM,DuFort CC,Ou G,Chang AC,Cassereau L,Tan SJ,Pickup MW,Lakins JN,Ye X,Davidson MW,Lammerding J,Odde DJ,Dunn AR,Weaver VM

44-625G was used in Immunofluorescence to study how analysis of the physical properties of tumor cells and integrin adhesion composition in biopsies may be predictive of patient outcome.

Thu Jun 01 00:00:00 UTC 2017

Angiopoietin-1 deficiency increases tumor metastasis in mice.

BMC cancer

Michael IP,Orebrand M,Lima M,Pereira B,Volpert O,Quaggin SE,Jeansson M

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Fri Aug 11 00:00:00 UTC 2017

Angiopoietin-1 deficiency increases tumor metastasis in mice.

BMC cancer

Michael IP,Orebrand M,Lima M,Pereira B,Volpert O,Quaggin SE,Jeansson M

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Fri Aug 11 00:00:00 UTC 2017

SCARA5 plays a critical role in the commitment of mesenchymal stem cells to adipogenesis.

Scientific reports

Lee H,Lee YJ,Choi H,Seok JW,Yoon BK,Kim D,Han JY,Lee Y,Kim HJ,Kim JW

44-625G was used in Western Blotting to demonstrate that SCARA5 is a positive regulator in adipocyte lineage commitment and early adipogenesis in mesenchymal stem cells.

Wed Nov 01 00:00:00 UTC 2017

Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression.

Nature medicine

Laklai H,Miroshnikova YA,Pickup MW,Collisson EA,Kim GE,Barrett AS,Hill RC,Lakins JN,Schlaepfer DD,Mouw JK,LeBleu VS,Roy N,Novitskiy SV,Johansen JS,Poli V,Kalluri R,Iacobuzio-Donahue CA,Wood LD,Hebrok M,Hansen K,Moses HL,Weaver VM

44-625G was used in immunohistochemistry to propose that epithelial tension and matricellular fibrosis contribute to the aggressiveness of SMAD4 mutant pancreatic tumors

Sun May 01 00:00:00 UTC 2016

Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression.

Nature medicine

44-625G was used in immunohistochemistry to propose that epithelial tension and matricellular fibrosis contribute to the aggressiveness of SMAD4 mutant pancreatic tumors

Sun May 01 00:00:00 UTC 2016

Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression.

Cancer discovery

Oudin MJ,Jonas O,Kosciuk T,Broye LC,Guido BC,Wyckoff J,Riquelme D,Lamar JM,Asokan SB,Whittaker C,Ma D,Langer R,Cima MJ,Wisinski KB,Hynes RO,Lauffenburger DA,Keely PJ,Bear JE,Gertler FB

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Immunofluorescence

Sun May 01 00:00:00 UTC 2016

HSP70 Inhibition Limits FAK-Dependent Invasion and Enhances the Response to Melanoma Treatment with BRAF Inhibitors.

Cancer research

Budina-Kolomets A,Webster MR,Leu JI,Jennis M,Krepler C,Guerrini A,Kossenkov AV,Xu W,Karakousis G,Schuchter L,Amaravadi RK,Wu H,Yin X,Liu Q,Lu Y,Mills GB,Xu X,George DL,Weeraratna AT,Murphy ME

44-625G was used in immunocytochemistry and western blot to determine limits on FAK-dependent invasion and enhancement of the response to melanoma treatment with BRAF inhibitors due to HSP70 inhibition

Sun May 01 00:00:00 UTC 2016

HSP70 Inhibition Limits FAK-Dependent Invasion and Enhances the Response to Melanoma Treatment with BRAF Inhibitors.

Cancer research

Budina-Kolomets A,Webster MR,Leu JI,Jennis M,Krepler C,Guerrini A,Kossenkov AV,Xu W,Karakousis G,Schuchter L,Amaravadi RK,Wu H,Yin X,Liu Q,Lu Y,Mills GB,Xu X,George DL,Weeraratna AT,Murphy ME

Sun May 01 00:00:00 UTC 2016

Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition.

The Journal of cell biology

Horton ER,Humphries JD,Stutchbury B,Jacquemet G,Ballestrem C,Barry ST,Humphries MJ

44-625G was used in immunocytochemistry and western blot to determine how modulation of FAK and Src adhesion signalling occurs without the need for adhesion complex composition

Mon Feb 01 00:00:00 UTC 2016

Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition.

The Journal of cell biology

Horton ER,Humphries JD,Stutchbury B,Jacquemet G,Ballestrem C,Barry ST,Humphries MJ

44-625G was used in immunocytochemistry and western blot to determine how modulation of FAK and Src adhesion signalling occurs without the need for adhesion complex composition

Mon Feb 01 00:00:00 UTC 2016

Quantitative Proteomics Reveals the Dynamics of Protein Phosphorylation in Human Bronchial Epithelial Cells during Internalization, Phagosomal Escape, and Intracellular Replication of Staphylococcus aureus.

Journal of proteome research

Richter E,Harms M,Ventz K,Nölker R,Fraunholz MJ,Mostertz J,Hochgräfe F

44625G was used in western blot to study phosphorylation events in Staphylococcus aureus-infected cells

Fri Dec 02 00:00:00 UTC 2016

Endothelin-1 mediated induction of extracellular matrix genes in strial marginal cells underlies strial pathology in Alport mice.

Hearing research

Meehan DT,Delimont D,Dufek B,Zallocchi M,Phillips G,Gratton MA,Cosgrove D

44625G was used in immunohistochemistry - frozen section to suggest a role for endothelin-1 activation of endothelin A receptors in glomerular pathogenesis

Tue Nov 01 00:00:00 UTC 2016

Krüppel-like factor 8 activates the transcription of C-X-C cytokine receptor type 4 to promote breast cancer cell invasion, transendothelial migration and metastasis.

Oncotarget

Mukherjee D,Lu H,Yu L,He C,Lahiri SK,Li T,Zhao J

44-625G was used in western blot to examine the contribution of KLF8, CXCR4, and FAK to breast cancer metastasis

Tue Apr 26 00:00:00 UTC 2016

β-Arrestin1 and Signal-transducing Adaptor Molecule 1 (STAM1) Cooperate to Promote Focal Adhesion Kinase Autophosphorylation and Chemotaxis via the Chemokine Receptor CXCR4.

The Journal of biological chemistry

Alekhina O,Marchese A

44625G was used in western blot to identify a role for STAM1 in CXCR4-dependent chemotaxis

Fri Dec 09 00:00:00 UTC 2016

Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin.

Nature communications

Long K,Moss L,Laursen L,Boulter L,Ffrench-Constant C

44-625G was used in immunohistochemistry to assess regulation of expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorinby integrin signaling

Wed Feb 03 00:00:00 UTC 2016

Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly.

Nature cell biology

Horton ER,Byron A,Askari JA,Ng DHJ,Millon-Frémillon A,Robertson J,Koper EJ,Paul NR,Warwood S,Knight D,Humphries JD,Humphries MJ

44-625G was used in immunocytochemistry to characterize the dynamics of a consensus integrin adhesome during adhesion complex assembly and disassembly

Tue Dec 01 00:00:00 UTC 2015

The adhesion GPCR GPR126 has distinct, domain-dependent functions in Schwann cell development mediated by interaction with laminin-211.

Neuron

Petersen SC,Luo R,Liebscher I,Giera S,Jeong SJ,Mogha A,Ghidinelli M,Feltri ML,Schöneberg T,Piao X,Monk KR

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Immunohistochemistry

Wed Feb 18 00:00:00 UTC 2015

Focal adhesion kinase is involved in rabies virus infection through its interaction with viral phosphoprotein P.

Journal of virology

Fouquet B,Nikolic J,Larrous F,Bourhy H,Wirblich C,Lagaudrière-Gesbert C,Blondel D

44-625G was used in Western Blotting to study the implication of focal adhesion kinase (FAK) in rabies virus infection and provide evidence that phosphoprotein (P) -FAK interaction has a proviral function.

Sun Feb 01 00:00:00 UTC 2015

Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li N,Mruk DD,Wong CK,Lee WM,Han D,Cheng CY

44-625G was used in immunohistochemistry to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis

Tue Sep 01 00:00:00 UTC 2015

Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li N,Mruk DD,Wong CK,Lee WM,Han D,Cheng CY

44-625G was used in immunohistochemistry to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis

Tue Sep 01 00:00:00 UTC 2015

A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Grossman H,Chuderland D,Ninio-Many L,Hasky N,Kaplan-Kraicer R,Shalgi R

44-625G was used in western blot to determine the role of miR-125a-3p and Fyn in granulosa cells.

Sat Aug 01 00:00:00 UTC 2015

Understanding the roles of FAK in cancer: inhibitors, genetic models, and new insights.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Yoon H,Dehart JP,Murphy JM,Lim ST

44-625G was used in Immunohistochemistry to discuss FAK inhibitors as well as genetic mouse models to provide mechanistic insights into FAK signaling and its potential in cancer therapy.

Sun Feb 01 00:00:00 UTC 2015

Understanding the roles of FAK in cancer: inhibitors, genetic models, and new insights.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Yoon H,Dehart JP,Murphy JM,Lim ST

44-625G was used in Immunohistochemistry to discuss FAK inhibitors as well as genetic mouse models to provide mechanistic insights into FAK signaling and its potential in cancer therapy.

Sun Feb 01 00:00:00 UTC 2015

Doxycycline inhibits leukemic cell migration via inhibition of matrix metalloproteinases and phosphorylation of focal adhesion kinase.

Molecular medicine reports

Wang C,Xiang R,Zhang X,Chen Y

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Tue Sep 01 00:00:00 UTC 2015

Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.

Nature communications

Robertson J,Jacquemet G,Byron A,Jones MC,Warwood S,Selley JN,Knight D,Humphries JD,Humphries MJ

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Fri Feb 13 00:00:00 UTC 2015

Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration.

Scientific reports

Wu JC,Chen YC,Kuo CT,Wenshin Yu H,Chen YQ,Chiou A,Kuo JC

44-625G was used in western blot to test if SRC, SHP1, and SHP2 target focal adhesions via their SH2 domains

Fri Dec 18 00:00:00 UTC 2015

Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration.

Scientific reports

Wu JC,Chen YC,Kuo CT,Wenshin Yu H,Chen YQ,Chiou A,Kuo JC

44-625G was used in western blot to test if SRC, SHP1, and SHP2 target focal adhesions via their SH2 domains

Fri Dec 18 00:00:00 UTC 2015

Macrolide analog F806 suppresses esophageal squamous cell carcinoma (ESCC) by blocking β1 integrin activation.

Oncotarget

Li LY,Jiang H,Xie YM,Liao LD,Cao HH,Xu XE,Chen B,Zeng FM,Zhang YL,Du ZP,Chen H,Huang W,Jia W,Zheng W,Xie JJ,Li EM,Xu LY

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Tue Jun 30 00:00:00 UTC 2015

Low molecular weight protein tyrosine phosphatase (LMWPTP) upregulation mediates malignant potential in colorectal cancer.

Oncotarget

Hoekstra E,Kodach LL,Das AM,Ruela-de-Sousa RR,Ferreira CV,Hardwick JC,van der Woude CJ,Peppelenbosch MP,Ten Hagen TL,Fuhler GM

44-625G was used in western blot determine the contribution of low molecular weight protein tyrosine phosphatase to colorectal cancer

Fri Apr 10 00:00:00 UTC 2015

A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin.

PloS one

Richter E,Harms M,Ventz K,Gierok P,Chilukoti RK,Hildebrandt JP,Mostertz J,Hochgräfe F

44-625G was used in western blot to use a multi-omics strategy to elucidate the cellular programs altered by Staphylococcus aureus alpha-toxin

Tue Feb 23 00:00:00 UTC 2016

Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate.

Cancer research

Rubashkin MG,Cassereau L,Bainer R,DuFort CC,Yui Y,Ou G,Paszek MJ,Davidson MW,Chen YY,Weaver VM

44-625G was used in immunocytochemistry to study the mechanism of the activation of focal adhesion protein vinculin by mechanical stress.

Mon Sep 01 00:00:00 UTC 2014

KLF8 and FAK cooperatively enrich the active MMP14 on the cell surface required for the metastatic progression of breast cancer.

Oncogene

Lu H,Hu L,Yu L,Wang X,Urvalek AM,Li T,Shen C,Mukherjee D,Lahiri SK,Wason MS,Zhao J

44-625G was used in western blot to show that KLF8 and MMP14 signaling promotes human breast cancer invasion and metastasis.

Thu May 29 00:00:00 UTC 2014

Herpes simplex virus type 2 glycoprotein H interacts with integrin αvβ3 to facilitate viral entry and calcium signaling in human genital tract epithelial cells.

Journal of virology

Cheshenko N,Trepanier JB,González PA,Eugenin EA,Jacobs WR,Herold BC

44-625G was used in western blot to test if integrin alphavbeta3 signaling promotes the release of intracellular calcium and contributes to viral entry and spread

Mon Sep 01 00:00:00 UTC 2014

MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells.

Oncoscience

Ninio-Many L,Grossman H,Levi M,Zilber S,Tsarfaty I,Shomron N,Tuvar A,Chuderland D,Stemmer SM,Ben-Aharon I,Shalgi R

44-625G was used in western blot to study miR-125a-3p in prostate cancer cells.

Fri Jan 16 00:00:00 UTC 2015

c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution.

American journal of physiology. Endocrinology and metabolism

Xiao X,Mruk DD,Cheng CY

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Immunofluorescence

Tue Jan 15 00:00:00 UTC 2013

microRNA-125a-3p reduces cell proliferation and migration by targeting Fyn.

Journal of cell science

Ninio-Many L,Grossman H,Shomron N,Chuderland D,Shalgi R

44-625G was used in Western Blotting to demonstrate that miR-125a-3p has an important role in the regulation of Fyn expression and of its signalling pathway.

Mon Jul 01 00:00:00 UTC 2013

Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes.

Endocrinology

Qian X,Mruk DD,Wong EW,Lie PP,Cheng CY

Wed May 01 00:00:00 UTC 2013

Intercellular adhesion molecule-2 is involved in apical ectoplasmic specialization dynamics during spermatogenesis in the rat.

The Journal of endocrinology

Xiao X,Cheng CY,Mruk DD

44-625G was used in western blot to assess the role of intercellular adhesion molecule-2 in the testis

Tue Jan 01 00:00:00 UTC 2013

Src-inducible association of CrkL with procaspase-8 promotes cell migration.

Cell adhesion & migration

Graf R,Barbero S,Keller N,Chen L,Uryu S,Schlaepfer D,Stupack D

44-625G was used in Western Blotting to elucidate how tyrosine kinases may act to coordinate the suppression of caspase-8 mediated apoptosis, while promoting cell invasion.

Wed Mar 19 00:00:00 UTC 2014

Colon cancer cells adopt an invasive phenotype without mesenchymal transition in 3-D but not 2-D culture upon combined stimulation with EGF and crypt growth factors.

BMC cancer

Ludwig K,Tse ES,Wang JY

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Thu May 02 00:00:00 UTC 2013

Kindlin-1 regulates mitotic spindle formation by interacting with integrins and Plk-1.

Nature communications

Patel H,Zich J,Serrels B,Rickman C,Hardwick KG,Frame MC,Brunton VG

44-625G was used in Immunohistochemistry-immunofluorescence to identify a novel cellular role for Kindlin-1 in ensuring mitotic spindle assembly and cellular survival that is controlled by phosphorylation via Polo-like kinase 1.

Mon Dec 30 00:00:00 UTC 2013

Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat.

Proceedings of the National Academy of Sciences of the United States of America

Lie PP,Mruk DD,Mok KW,Su L,Lee WM,Cheng CY

Tue Jul 31 00:00:00 UTC 2012

Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat.

Proceedings of the National Academy of Sciences of the United States of America

Lie PP,Mruk DD,Mok KW,Su L,Lee WM,Cheng CY

Tue Jul 31 00:00:00 UTC 2012

FAK promotes recruitment of talin to nascent adhesions to control cell motility.

The Journal of cell biology

Lawson C,Lim ST,Uryu S,Chen XL,Calderwood DA,Schlaepfer DD

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Mon Jan 23 00:00:00 UTC 2012

Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression.

The Journal of cell biology

Lim ST,Miller NL,Chen XL,Tancioni I,Walsh CT,Lawson C,Uryu S,Weis SM,Cheresh DA,Schlaepfer DD

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Mon Jun 25 00:00:00 UTC 2012

Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression.

The Journal of cell biology

Lim ST,Miller NL,Chen XL,Tancioni I,Walsh CT,Lawson C,Uryu S,Weis SM,Cheresh DA,Schlaepfer DD

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Mon Jun 25 00:00:00 UTC 2012

Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Orlando C,Ster J,Gerber U,Fawcett JW,Raineteau O

44-625G was used in Immunocytochemistry-immunoflourescence to assess the effect of chondroitinase ABC-mediated digestion of chondroitin sulphate proteoglycans on dendritic spine dynamics.

Wed Dec 12 00:00:00 UTC 2012

Filamin C plays an essential role in the maintenance of the structural integrity of cardiac and skeletal muscles, revealed by the medaka mutant zacro.

Developmental biology

Fujita M,Mitsuhashi H,Isogai S,Nakata T,Kawakami A,Nonaka I,Noguchi S,Hayashi YK,Nishino I,Kudo A

44-625G was used in Immunohistochemistry-wholemount to suggest that filamin C plays a role in the maintenance of the structural integrity of cardiac and skeletal muscles.

Sun Jan 01 00:00:00 UTC 2012

Cortactin as a target for FAK in the regulation of focal adhesion dynamics.

PloS one

Tomar A,Lawson C,Ghassemian M,Schlaepfer DD

44-625G was used in western blot to investigate the consequences of FAK and cortactin interactions

Thu Feb 07 00:00:00 UTC 2013

Specific β-containing integrins exert differential control on proliferation and two-dimensional collective cell migration in mammary epithelial cells.

The Journal of biological chemistry

Jeanes AI,Wang P,Moreno-Layseca P,Paul N,Cheung J,Tsang R,Akhtar N,Foster FM,Brennan K,Streuli CH

Fri Jul 13 00:00:00 UTC 2012

Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.

PloS one

Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC

44-625G was used in immunocytochemistry to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions

Wed Jul 21 00:00:00 UTC 2010

A FAK-p120RasGAP-p190RhoGAP complex regulates polarity in migrating cells.

Journal of cell science

Tomar A,Lim ST,Lim Y,Schlaepfer DD

Published figure using Phospho-FAK (Tyr397) monoclonal antibody (Product # 44-625G) in Western Blot

Mon Jun 01 00:00:00 UTC 2009

An adaptor role for cytoplasmic Sam68 in modulating Src activity during cell polarization.

Molecular and cellular biology

Huot ME,Brown CM,Lamarche-Vane N,Richard S

44-625G was used in western blot to demonstrate that Sam68-deficient cells exhibit loss of cell polarity and cell migration

Wed Apr 01 00:00:00 UTC 2009

Increased expression and phosphorylation of focal adhesion kinase correlates with dysfunction in the volume-overloaded human heart.

Clinical science (London, England : 1979)

Lopes MM,Ribeiro GC,Tornatore TF,Clemente CF,Teixeira VP,Franchini KG

44-625G was used in immunohistochemistry to investigate if FAK expression and phosphorylation is altered in the volume-overloaded human heart

Wed Aug 01 00:00:00 UTC 2007

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 UTC 2006

Analyzing FAK and Pyk2 in early integrin signaling events.

Current protocols in cell biology

Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D

Sat Apr 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; EC 2.7.10.2; FADK 1; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

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Gene Aliases: CG10023; CT28129; DFAK; DFak56; Dmel\CG10023; Dmel_CG10023; DmFAK; FADK; FAK; FAK1; Fak56; Fak56D; FAK65D; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2; XELAEV_18032408mg; XPFAK

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UniProt ID: (Human) Q05397, (Mouse) P34152, (Rat) O35346, (Xenopus) Q91738

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Entrez Gene ID: (Fruit fly) 37233, (Human) 5747, (Chicken) 396416, (Mouse) 14083, (Rat) 25614, (Xenopus) 399286

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Function(s)

protein tyrosine kinase activity signal transducer activity ATP binding actin binding protein kinase activity non-membrane spanning protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding JUN kinase binding protein kinase binding SH2 domain binding identical protein binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding

Process(es)

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Phospho-FAK (Tyr397) Monoclonal Antibody (141-9)

Phospho-FAK (Tyr397) Antibody (44-625G) in ICC/IF

Product Details

44-625G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

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Class

Type

Clone

Immunogen

Conjugate

Form

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