Invitrogen

Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (2H74L24)

Advanced Verification
8 Published Figures
9 References
View all (101) FAK antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

700013

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 5 publications 5 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
-

Immunocytochemistry (ICC/IF)

1:100
View 2 publications 2 publications

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Xenopus

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

2H74L24

Immunogen

A peptide corresponding to amino acids 573-582 of Q05397.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532268

Product Specific Information

This antibody is predicted to react with chimpanzee, Rhesus monkey, mouse, rat, chicken, Xenopus, zebrafish and Macaque based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

View more View less

Gene Aliases: FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71; PTK2

View more View less

UniProt ID: (Human) Q05397

View more View less

Entrez Gene ID: (Human) 5747

View more View less

Function(s)
actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding
Process(es)
MAPK cascade microtubule cytoskeleton organization angiogenesis vasculogenesis neuron migration placenta development regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis cellular component disassembly involved in execution phase of apoptosis signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway axon guidance positive regulation of cell proliferation regulation of cell shape embryo development regulation of endothelial cell migration regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion establishment of cell polarity extracellular matrix organization positive regulation of cell migration regulation of cell adhesion mediated by integrin netrin-activated signaling pathway peptidyl-tyrosine autophosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process regulation of GTPase activity endothelial cell migration positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity innate immune response regulation of osteoblast differentiation positive regulation of protein kinase activity negative regulation of organ growth protein autophosphorylation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway cell motility negative regulation of axonogenesis regulation of cytoskeleton organization regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly growth hormone receptor signaling pathway regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support