Invitrogen

Phospho-FOXO1/FOXO3 (Ser322, Ser325) Polyclonal Antibody

View all (2) FOXO1/FOXO3 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-40217

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (IHC)

1:100-1:300
-

ELISA (ELISA)

1:5,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthesized peptide derived from human FoxO1/3 around the phosphorylation site of S322/S325.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.5% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576790

Product Specific Information

This antibody is predicted to react with human and rat based on sequence homology.

Target Information

a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AF6q21 protein; AFX; AFX1; forkhead box O1a; forkhead box O1A (rhabdomyosarcoma); forkhead box O3A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead box protein O3; forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma-like 1; forkhead protein 1; forkhead protein FKHR2; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOXO4; MGC120490; MLLT7

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Gene Aliases: 1110048B16Rik; 2010203A17Rik; AF6q21; Afxh; AI876417; C76856; FKH1; FKHR; Fkhr1; Fkhr2; FKHRL1; FKHRL1P2; FOXO1; FOXO1A; FOXO2; FOXO3; FOXO3A

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UniProt ID: (Human) Q12778, (Human) O43524, (Rat) G3V7R4, (Mouse) Q9R1E0, (Mouse) Q9WVH4

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Entrez Gene ID: (Human) 2308, (Human) 2309, (Rat) 84482, (Mouse) 56458, (Mouse) 56484, (Rat) 294515

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, transcription factor binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding protein binding beta-catenin binding ubiquitin protein ligase binding sequence-specific DNA binding protein phosphatase 2A binding core promoter binding transcription cofactor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein kinase binding chromatin DNA binding transcription factor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ovulation from ovarian follicle initiation of primordial ovarian follicle growth antral ovarian follicle growth oocyte maturation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter regulation of translation apoptotic process anatomical structure morphogenesis cell differentiation DNA damage response, signal transduction by p53 class mediator cellular response to oxidative stress glucose homeostasis positive regulation of neuron apoptotic process positive regulation of erythrocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance extrinsic apoptotic signaling pathway in absence of ligand regulation of neural precursor cell proliferation regulation of reactive oxygen species metabolic process blood vessel development autophagy cellular response to DNA damage stimulus insulin receptor signaling pathway cellular response to starvation positive regulation of autophagy cellular response to insulin stimulus negative regulation of stress-activated MAPK cascade regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter regulation of cell proliferation positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of fat cell differentiation positive regulation of gluconeogenesis positive regulation of protein catabolic process cellular response to hyperoxia cellular response to nitric oxide regulation of nucleic acid-templated transcription tumor necrosis factor-mediated signaling pathway temperature homeostasis cellular glucose homeostasis protein acetylation endocrine pancreas development fat cell differentiation negative regulation of transcription, DNA-templated enamel mineralization cellular response to hydrogen peroxide cellular response to cold cellular response to dexamethasone stimulus response to fluoride regulation of energy homeostasis
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