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Phospho-GAB1 (Tyr627) Antibody (44-568G) in WB
Stimulation and Antibody-Peptide Competition. Lysates of NIH3T3 cells untreated (1) or treated with 50 ng/mL PDGF for 15 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and then incubated with the Gab1 (pY627) antibody for two hours at room temperature in a 3% BSA-TBST buffer, follo... View More
Product Details
44-568G
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Gab1 that contains tyrosine 627. The sequence is conserved in mouse.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533682
Target Information
Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet-derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met), and epidermal growth factor receptor (EGF), as well as in response to cell-cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3-kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GRB2-associated binder 1; GRB2-associated binding protein 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; OTTHUMP00000220263
Gene Aliases: AA408973; AW107238; GAB1
UniProt ID: (Human) Q13480, (Mouse) Q9QYY0
Entrez Gene ID: (Human) 2549, (Mouse) 14388
response to oxidative stress
epidermal growth factor receptor signaling pathway
activation of JUN kinase activity
heart development
cell proliferation
insulin receptor signaling pathway
epidermis development
regulation of phosphatidylinositol 3-kinase signaling
regulation of cell migration
phosphatidylinositol-3-phosphate biosynthetic process
ERBB2 signaling pathway
phosphatidylinositol phosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
labyrinthine layer development
interleukin-6-mediated signaling pathway
activation of MAPK activity
signal transduction
positive regulation of MAPK cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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