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Invitrogen
Phospho-GRF-1 (Tyr1105) Polyclonal Antibody
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Product Details
PA5-36713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1105
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2553676
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 172 kDa.
Purity is >95% by SDS-PAGE.
Target Information
RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GAP-associated protein p190; glucocorticoid receptor DNA binding factor 1; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; MGC10745; P190 RhoGAP; p190-A; Rho GAP p190A; Rho GTPase-activating protein 35
Gene Aliases: 6430596G11Rik; AI841135; ARHGAP35; GRF-1; GRF1; GRLF1; KIAA1722; mKIAA1722; P190-A; P190A; p190ARHOGAP; p190RhoGAP
UniProt ID: (Human) Q9NRY4, (Rat) P81128, (Mouse) Q91YM2
Entrez Gene ID: (Human) 2909, (Rat) 306400, (Mouse) 232906
negative regulation of transcription from RNA polymerase II promoter
neural tube closure
transcription, DNA-templated
regulation of transcription, DNA-templated
small GTPase mediated signal transduction
regulation of cell shape
positive regulation of neuron projection development
forebrain development
negative regulation of Rho protein signal transduction
camera-type eye development
negative regulation of vascular permeability
positive regulation of GTPase activity
negative regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
integrin-mediated signaling pathway
regulation of actin polymerization or depolymerization
positive regulation of cilium assembly
regulation of small GTPase mediated signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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