Invitrogen

Phospho-GSK3 alpha/beta (Ser21, Ser9) Recombinant Rabbit Monoclonal Antibody (2J1P4)

View all (17) GSK3 alpha/beta antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-51516

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2J1P4

Immunogen

A synthetic phosphorylated peptide around S21&S9 of human GSK3 alpha/GSK3 beta (NP_063937.2/NP_002084.2).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.83 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3093953

Product Specific Information

Immunogen sequence: TSSFA

Target Information

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B

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Gene Aliases: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; Gsk3a; Gsk3b

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UniProt ID: (Mouse) Q2NL51, (Mouse) Q9WV60

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Entrez Gene ID: (Mouse) 606496, (Mouse) 56637

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase A catalytic subunit binding tau-protein kinase activity RNA polymerase II transcription factor binding p53 binding integrin binding beta-catenin binding transcription factor binding protein kinase binding ubiquitin protein ligase binding ionotropic glutamate receptor binding tau protein binding NF-kappaB binding
Process(es)
re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion carbohydrate metabolic process glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation protein export from nucleus ER overload response cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development nervous system development axonogenesis myoblast fusion circadian rhythm cell proliferation organ morphogenesis negative regulation of signal transduction negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation regulation of neuron projection development negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation Wnt signaling pathway phosphorylation cell migration peptidyl-serine phosphorylation hippocampus development establishment of cell polarity cell differentiation negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation protein localization to microtubule intracellular signal transduction cellular response to interleukin-3 positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity rhythmic process negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway superior temporal gyrus development cellular response to mechanical stimulus extrinsic apoptotic signaling pathway in absence of ligand positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of stem cell differentiation regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis insulin receptor signaling pathway positive regulation of cAMP biosynthetic process negative regulation of TOR signaling cellular response to insulin stimulus proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of heart contraction negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of transferase activity negative regulation of cell growth involved in cardiac muscle cell development cellular response to lithium ion cellular response to organic cyclic compound positive regulation of adrenergic receptor signaling pathway regulation of mitophagy positive regulation of protein targeting to mitochondrion positive regulation of glycogen (starch) synthase activity
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