Invitrogen

Phospho-GSK3B (Ser9) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA1-4688

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 2 publications 2 publications
Product Specifications

Species Reactivity

Mouse, Rat

Published species

Amphibian, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3B conjugated to KLH
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2115231

Product Specific Information

This antibody is predicted to react with human, mouse, chicken, bovine, canine, non-human primate, zebrafish and Xenopus based on 100% sequence homology.

In Western blot, this antibody is specific for the ~46kDa GSK3 beta protein phosphorylated at Ser9. It also weakly labels the ~51 kDa GSK3a band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Target Information

GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; pp37; Serine/threonine-protein kinase GSK3B

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Gene Aliases: 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; Gsk3b

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UniProt ID: (Mouse) Q9WV60, (Rat) P18266

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Entrez Gene ID: (Mouse) 56637, (Rat) 84027

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Function(s)
nucleotide binding RNA polymerase II transcription factor binding p53 binding protein kinase activity protein serine/threonine kinase activity integrin binding protein binding ATP binding beta-catenin binding transcription factor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding ionotropic glutamate receptor binding tau protein binding tau-protein kinase activity NF-kappaB binding
Process(es)
re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion carbohydrate metabolic process glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation protein export from nucleus ER overload response cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development nervous system development axonogenesis myoblast fusion circadian rhythm cell proliferation organ morphogenesis negative regulation of signal transduction negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation regulation of neuron projection development negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation Wnt signaling pathway phosphorylation cell migration peptidyl-serine phosphorylation hippocampus development establishment of cell polarity cell differentiation negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation protein localization to microtubule intracellular signal transduction cellular response to interleukin-3 positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity rhythmic process negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway superior temporal gyrus development cellular response to mechanical stimulus extrinsic apoptotic signaling pathway in absence of ligand positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of stem cell differentiation response to lithium ion response to drug
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