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          • Primary Antibodies ›
          • GSK3B Antibodies

          Invitrogen

          Phospho-GSK3B (Ser9) Polyclonal Antibody

          Advanced Verification
          View all (84) GSK3B antibodies

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          Datasheet
          Protocols
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          Cite Phospho-GSK3B (Ser9) Polyclonal Antibody

          • Antibody Testing Data (15)
          • Advanced Verification (1)
          Phospho-GSK3B (Ser9) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-GSK3B (Ser9) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 16

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          Phospho-GSK3B (Ser9) Antibody (PA5-104555) in ICC/IF

          Immunofluorescence analysis of Glycogen synthase kinase-3 beta was performed using 70% confluent log phase HeLa and HeLa cells treated with Calyculin A (100 nm for 60 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Phospho-GSK3B (Ser9) Polyclon... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-GSK3B (Ser9) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-GSK3B (Ser9) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-GSK3B (Ser9) Antibody in Western Blot (WB)
          Phospho-GSK3B (Ser9) Antibody in Western Blot (WB)
          Phospho-GSK3B (Ser9) Antibody in Western Blot (WB)
          Phospho-GSK3B (Ser9) Antibody in Western Blot (WB)
          Phospho-GSK3B (Ser9) Antibody

          Product Details

          PA5-104555

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -

          Immunoprecipitation (IP)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human GSK3B(Accession P49841), corresponding to amino acid residues around phosphorylated Ser9.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          sequential chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2816030

          Product Specific Information

          Antibody detects endogenous levels of GSK3 beta only when phosphorylated at Serine 9.

          Target Information

          GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3beta isoform; pp37; Serine/threonine-protein kinase GSK3B

          View more View less

          Gene Aliases: 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; GSK3B

          View more View less

          UniProt ID: (Human) P49841, (Mouse) Q9WV60, (Rat) P18266

          View more View less

          Entrez Gene ID: (Human) 2932, (Mouse) 56637, (Rat) 84027

          View more View less

          Function(s)
          RNA polymerase II transcription factor binding p53 binding protein kinase activity protein serine/threonine kinase activity integrin binding protein binding ATP binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding ionotropic glutamate receptor binding tau protein binding tau-protein kinase activity NF-kappaB binding nucleotide binding transcription factor binding transferase activity transferase activity, transferring phosphorus-containing groups
          Process(es)
          re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion carbohydrate metabolic process glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation protein export from nucleus ER overload response cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development nervous system development axonogenesis myoblast fusion circadian rhythm cell proliferation organ morphogenesis negative regulation of signal transduction negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation regulation of neuron projection development negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation Wnt signaling pathway phosphorylation cell migration peptidyl-serine phosphorylation hippocampus development establishment of cell polarity cell differentiation negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation protein localization to microtubule intracellular signal transduction cellular response to interleukin-3 positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity rhythmic process negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway superior temporal gyrus development cellular response to mechanical stimulus extrinsic apoptotic signaling pathway in absence of ligand positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of stem cell differentiation dopamine receptor signaling pathway aging positive regulation of mitochondrial membrane potential response to activity peptidyl-threonine phosphorylation response to estradiol response to insulin negative regulation of smooth muscle cell apoptotic process cellular response to hepatocyte growth factor stimulus cellular response to erythropoietin response to drug proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process negative regulation of glycogen biosynthetic process positive regulation of protein catabolic process positive regulation of smooth muscle cell proliferation negative regulation of nitric-oxide synthase activity intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to iron(II) ion cellular response to lithium ion response to epinephrine negative regulation of canonical Wnt signaling pathway chemical synaptic transmission, postsynaptic regulation of cellular response to heat negative regulation of protein localization to nucleus positive regulation of neuron death response to L-glutamate cellular response to dopamine negative regulation of dopaminergic neuron differentiation beta-catenin destruction complex assembly beta-catenin destruction complex disassembly response to insulin-like growth factor stimulus response to ultrasound response to angiotensin negative regulation of type B pancreatic cell development positive regulation of excitatory postsynaptic potential negative regulation of glycogen (starch) synthase activity positive regulation of DNA biosynthetic process negative regulation of neuron migration response to lithium ion
          It has to be done as per old AB suggested Products section.
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