Invitrogen

Phospho-GYS1 (Ser641, Ser645) Polyclonal Antibody

Phospho-GYS1 (Ser641, Ser645) Antibody in Western Blot (WB)

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Phospho-GYS1 (Ser641, Ser645) Antibody (44-1092G) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), U-87 MG treated with Cobalt chloride (150uM for 24hrs) (Lane 2), L6 (Lane 3), L6 treated with Insulin (10nM for 24hrs) (Lane 4), MCF-7 (Lane 5) and MCF-7 treated with Cobalt chloride (Lane 6). The blot was probed with Anti-Phospho-GYS1 (Ser641, Ser645) Antibody (Product # 44-1092G, 1:500 dilution) and detected by ... View More

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Product Details

44-1092G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Glycogen Synthase that contains serine 641 and serine 645. The sequence is conserved in human, mouse and rat.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533572

Target Information

Glycogen Synthase 1 (GYS1) catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fibroblast growth factor 21 improves hepatic insulin sensitivity by inhibiting mammalian target of rapamycin complex 1 in mice.

Hepatology (Baltimore, Md.)

Gong Q,Hu Z,Zhang F,Cui A,Chen X,Jiang H,Gao J,Chen X,Han Y,Liang Q,Ye D,Shi L,Chin YE,Wang Y,Xiao H,Guo F,Liu Y,Zang M,Xu A,Li Y

44-1092G was used in western blot to assess the inhibition of mammalian target of rapamycin complex 1 in mice due to fibroblast growth factor 21 improving hepatic insulin sensitivity

Mon Aug 01 00:00:00 UTC 2016

Accelerated recovery of mitochondrial membrane potential by GSK-3β inactivation affords cardiomyocytes protection from oxidant-induced necrosis.

PloS one

Sunaga D,Tanno M,Kuno A,Ishikawa S,Ogasawara M,Yano T,Miki T,Miura T

44-1092G was used in western blot to identify the role of mKATP channel activation in mitochondrial membrane potential and cell death

Mon Jul 06 00:00:00 UTC 2015

Translocation of glycogen synthase kinase-3β (GSK-3β), a trigger of permeability transition, is kinase activity-dependent and mediated by interaction with voltage-dependent anion channel 2 (VDAC2).

The Journal of biological chemistry

Tanno M,Kuno A,Ishikawa S,Miki T,Kouzu H,Yano T,Murase H,Tobisawa T,Ogasawara M,Horio Y,Miura T

44-1092G was used in western blot to examine the mechanism of translocation in regards to glycogen synthase kinase-3 protein

Fri Oct 17 00:00:00 UTC 2014

Glycogen synthase kinase-3 inactivation is not required for ischemic preconditioning or postconditioning in the mouse.

Circulation research

Nishino Y,Webb IG,Davidson SM,Ahmed AI,Clark JE,Jacquet S,Shah AM,Miura T,Yellon DM,Avkiran M,Marber MS

44-1092G was used in Western Blotting to show that the inhibition of glycogen synthase kinase-3beta is unlikely to be the key determinant of cardioprotective signalling in either preconditioning or postconditioning in the mouse.

Fri Aug 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: EC 2.4.1.11; Glycogen [starch] synthase, muscle; glycogen synthase; Glycogen synthase 1; glycogen synthase 1 (muscle); glycogen synthase 3, brain

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Gene Aliases: GSY; GYS; GYS1; Gys3; MGS

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UniProt ID: (Human) P13807, (Rat) A2RRU1, (Mouse) Q9Z1E4

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Entrez Gene ID: (Human) 2997, (Rat) 690987, (Mouse) 14936

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Function(s)

glycogen (starch) synthase activity protein binding glucose binding protein kinase binding glycogen synthase activity, transferring glucose-1-phosphate catalytic activity transferase activity transferase activity, transferring glycosyl groups

Process(es)

glycogen metabolic process glycogen biosynthetic process heart development metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-GYS1 (Ser641, Ser645) Polyclonal Antibody

Phospho-GYS1 (Ser641, Ser645) Antibody (44-1092G) in WB

Product Details

44-1092G

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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