Invitrogen

Phospho-GluR1 (Ser845) Polyclonal Antibody

2 Published Figures

5 References

View all (44) GluR1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

Phospho-GluR1 (Ser845) Antibody in Western Blot (WB)

FIGURE: 1 / 3

Phospho-GluR1 (Ser845) Antibody (OPA1-04118) in WB

Western blot of GluR1 in rat hippocampal lysate showing specific immunolabeling of a ~100 kDa band corresponding to Phospho-GluR1 (Ser845) polyclonal antibody (Product # OPA1-04118) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).

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Product Details

OPA1-04118

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 of rat GRIA1 conjugated to KLH

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325368

Product Specific Information

This antibody is predicted to react with human, mouse and non-human primate based on 100% sequence homology.

In Western blot, this antibody detects a ~100 kDa protein representing phospho-GluR1 (pSer845) in rat brain lysate. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Target Information

GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GSK3α, not GSK3β, drives hippocampal NMDAR-dependent LTD via tau-mediated spine anchoring.

The EMBO journal

Draffin JE,Sánchez-Castillo C,Fernández-Rodrigo A,Sánchez-Sáez X,Ávila J,Wagner FF,Esteban JA

OPA1-04118 was used in Western Blotting to investigate the importance of isoform-specific functions of GSK3 in synaptic plasticity.

Fri Jan 15 00:00:00 UTC 2021

KIF13A drives AMPA receptor synaptic delivery for long-term potentiation via endosomal remodeling.

The Journal of cell biology

Gutiérrez Y,López-García S,Lario A,Gutiérrez-Eisman S,Delevoye C,Esteban JA

Published figure using Phospho-GluR1 (Ser845) polyclonal antibody (Product # OPA1-04118) in Western Blot

Mon Jun 07 00:00:00 UTC 2021

Sex-Specific Anxiety and Prefrontal Cortex Glutamatergic Dysregulation Are Long-Term Consequences of Pre-and Postnatal Exposure to Hypercaloric Diet in a Rat Model.

Nutrients

Rivera P,Tovar R,Ramírez-López MT,Navarro JA,Vargas A,Suárez J,Fonseca FR

OPA1-04118 was used in Western Blotting to conclude that a hypercaloric maternal diet induces sex-dependent anxiety, associated with alterations in both glutamatergic and cannabinoid signaling in the prefrontal cortex, which are accentuated with the continuation of the palatable diet during the life of the offspring.

Fri Jun 19 00:00:00 UTC 2020

Sex-Specific Anxiety and Prefrontal Cortex Glutamatergic Dysregulation Are Long-Term Consequences of Pre-and Postnatal Exposure to Hypercaloric Diet in a Rat Model.

Nutrients

Rivera P,Tovar R,Ramírez-López MT,Navarro JA,Vargas A,Suárez J,Fonseca FR

Fri Jun 19 00:00:00 UTC 2020

Cocaine-induced behavioral sensitization decreases the expression of endocannabinoid signaling-related proteins in the mouse hippocampus.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology

Blanco E,Galeano P,Palomino A,Pavón FJ,Rivera P,Serrano A,Alen F,Rubio L,Vargas A,Castilla-Ortega E,Decara J,Bilbao A,de Fonseca FR,Suárez J

OPA1-04118 was used in western blot to determine if the hippocampal gene/protein expression of relevant glutamate signaling components, cannabinoid type 1 receptor, and endocannabinoid metabolic enzymes were altered following acute and/or repeated cocaine administration

Tue Mar 01 00:00:00 UTC 2016

Attenuation of cocaine-induced conditioned locomotion is associated with altered expression of hippocampal glutamate receptors in mice lacking LPA1 receptors.

Psychopharmacology

Blanco E,Bilbao A,Luque-Rojas MJ,Palomino A,Bermúdez-Silva FJ,Suárez J,Santín LJ,Estivill-Torrús G,Gutiérrez A,Campos-Sandoval JA,Alonso-Carrión FJ,Márquez J,de Fonseca FR

OPA1-04118 was used in western blot to investigate the contribution of LPA1 receptors to drug abuse

Thu Mar 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: AI853806; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GluR K1; GluR-1; GluR-A; GluR-K1; GluR1; GluRK1; glutamate receptor; Glutamate receptor 1; glutamate receptor A; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GPRC1A; Gria 1; GRM1A; HGBR1; HIPA; MGC13325; MGC133252; NMDA1; NMDAR1; NR1; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; RP11-350O14.1

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Gene Aliases: 2900051M01Rik; Glr-1; Glr1; GluA1; Glur-1; GluR-A; gluR-K1; Glur1; GluRA; Gria1; HIPA1

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UniProt ID: (Rat) P19490, (Mouse) P23818

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Entrez Gene ID: (Rat) 50592, (Mouse) 14799

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Function(s)

G-protein alpha-subunit binding ionotropic glutamate receptor activity AMPA glutamate receptor activity extracellular-glutamate-gated ion channel activity protein binding adenylate cyclase binding protein kinase binding protein domain specific binding PDZ domain binding small GTPase binding myosin V binding G-protein beta-subunit binding beta-2 adrenergic receptor binding identical protein binding protein homodimerization activity protein kinase A binding receptor activity ion channel activity

Process(es)

regulation of receptor recycling chemical synaptic transmission long-term memory response to toxic substance response to lithium ion response to organic cyclic compound neuronal action potential spinal cord development receptor internalization response to estradiol ion transmembrane transport ionotropic glutamate receptor signaling pathway synaptic transmission, glutamatergic response to cocaine response to drug response to peptide hormone positive regulation of membrane potential response to arsenic-containing substance regulation of synaptic plasticity modulation of synaptic transmission positive regulation of synaptic transmission response to electrical stimulus long term synaptic depression response to fungicide cellular response to amino acid stimulus cellular response to dsRNA cellular response to growth factor stimulus cellular response to peptide hormone stimulus cellular response to organic cyclic compound cellular response to amine stimulus transport ion transport

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Phospho-GluR1 (Ser845) Polyclonal Antibody

Phospho-GluR1 (Ser845) Antibody (OPA1-04118) in WB

Product Details

OPA1-04118

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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