Invitrogen

Phospho-HDAC4/HDAC5/HDAC9 (Ser246, Ser259, Ser220) Recombinant Rabbit Monoclonal Antibody (8H4J9)

View all (2) HDAC4/HDAC5/HDAC9 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-51562

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8H4J9

Immunogen

A phospho synthetic peptide corresponding to residues surrounding S246&S259&S220 of Human HDAC4&HDAC5-S259&HDAC9.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.84 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3094411

Target Information

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Antigen NY-CO-9; DKFZp779K1053; HD4; HD5; HD7; HD7b; HD9; hdac-9; Histone deacetylase 4; histone deacetylase 4/5-related protein; Histone deacetylase 5; Histone deacetylase 7B; Histone deacetylase 9; histone deacetylase A; histone deacetylase mHDA1; Histone deacetylase-related protein; MEF-2 interacting transcription repressor (MITR) protein; MEF2-interacting transcription repressor MITR

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Gene Aliases: 4932408F19Rik; AHO3; AI047285; AI426555; AV022454; BDMR; D030072B18Rik; HA6116; HD4; HD5; HD7; HD7B; HD9; HDAC; HDAC-4; HDAC-A; HDAC4; HDAC5; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDACA; HDRP; KIAA0288; KIAA0600; KIAA0744; mHDA1; MITR; mKIAA0600; mKIAA0744; NY-CO-9; RGD1310748; RGD1563092

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UniProt ID: (Human) P56524, (Human) Q9UQL6, (Human) Q9UKV0, (Rat) Q99P99, (Mouse) Q6NZM9, (Mouse) Q99N13

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Entrez Gene ID: (Human) 9759, (Human) 10014, (Human) 9734, (Rat) 363287, (Mouse) 208727, (Rat) 84580, (Mouse) 15184, (Rat) 687001, (Mouse) 79221

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Function(s)
RNA polymerase III transcription factor binding core promoter binding chromatin binding transcription corepressor activity histone deacetylase activity protein binding transcription factor binding zinc ion binding protein kinase binding potassium ion binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity activating transcription factor binding histone deacetylase binding sequence-specific DNA binding transcription regulatory region DNA binding repressing transcription factor binding protein kinase C binding metal ion binding molecular_function DNA binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific) hydrolase activity histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter osteoblast development chromatin organization transcription from RNA polymerase II promoter protein deacetylation heart development regulation of myotube differentiation negative regulation of myotube differentiation response to activity histone deacetylation neuron differentiation multicellular organismal response to stress regulation of gene expression, epigenetic response to cocaine response to drug regulation of protein binding negative regulation of osteoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of skeletal muscle fiber development positive regulation of sequence-specific DNA binding transcription factor activity regulation of striated muscle cell differentiation renal tubule morphogenesis cellular response to lipopolysaccharide cellular response to fluid shear stress negative regulation of cell migration involved in sprouting angiogenesis positive regulation of neural precursor cell proliferation regulation of histone H3-K9 acetylation positive regulation of stem cell proliferation response to amphetamine transcription, DNA-templated biological_process cellular response to insulin stimulus peptidyl-lysine deacetylation histone H3-K9 modification histone H3 deacetylation histone H4 deacetylation positive regulation of cell migration involved in sprouting angiogenesis skeletal system development chromatin remodeling inflammatory response nervous system development positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of lamellipodium assembly regulation of cardiac muscle contraction by calcium ion signaling response to denervation involved in regulation of muscle adaptation cardiac muscle hypertrophy in response to stress positive regulation of smooth muscle cell migration chromatin modification B cell differentiation positive regulation of protein sumoylation B cell activation negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of neuron apoptotic process negative regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of smooth muscle cell proliferation response to interleukin-1 cellular response to mechanical stimulus cellular response to tumor necrosis factor cellular response to parathyroid hormone stimulus positive regulation of reactive oxygen species biosynthetic process regulation of transcription, DNA-templated negative regulation of striated muscle tissue development negative regulation of gene expression chromatin silencing
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