Invitrogen

Phospho-HDAC5/HDAC9 (Ser259, Ser220) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-40215

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (IHC)

1:100-1:300
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthesized peptide derived from human HDAC5/9 around the phosphorylation site of S259/220.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576598

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Target Information

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Antigen NY-CO-9; HD5; HD7; HD7b; HD9; hdac-9; histone deacetylase 4; histone deacetylase 4/5-related protein; Histone deacetylase 5; Histone deacetylase 7B; Histone deacetylase 9; histone deacetylase mHDA1; Histone deacetylase-related protein; MEF-2 interacting transcription repressor (MITR) protein; MEF2-interacting transcription repressor MITR

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Gene Aliases: AI426555; AV022454; D030072B18Rik; HD5; HD7; HD7B; HD9; HDAC; Hdac4; HDAC5; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; KIAA0600; KIAA0744; mHDA1; MITR; mKIAA0600; mKIAA0744; NY-CO-9

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UniProt ID: (Human) Q9UQL6, (Human) Q9UKV0, (Mouse) Q99N13

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Entrez Gene ID: (Human) 10014, (Human) 9734, (Mouse) 15184, (Mouse) 79221

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Function(s)
RNA polymerase III transcription factor binding core promoter binding chromatin binding histone deacetylase activity protein kinase C binding protein binding transcription factor binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity histone deacetylase binding transcription regulatory region DNA binding metal ion binding repressing transcription factor binding transcription corepressor activity core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding hydrolase activity protein kinase binding histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)
Process(es)
negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated inflammatory response nervous system development heart development chromatin modification histone deacetylation B cell differentiation cellular response to insulin stimulus peptidyl-lysine deacetylation B cell activation negative regulation of striated muscle tissue development negative regulation of transcription, DNA-templated regulation of skeletal muscle fiber development regulation of striated muscle cell differentiation histone H3 deacetylation histone H4 deacetylation positive regulation of cell migration involved in sprouting angiogenesis osteoblast development transcription from RNA polymerase II promoter protein deacetylation negative regulation of gene expression regulation of myotube differentiation negative regulation of myotube differentiation multicellular organismal response to stress regulation of gene expression, epigenetic response to cocaine response to drug regulation of protein binding negative regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of sequence-specific DNA binding transcription factor activity renal tubule morphogenesis cellular response to fluid shear stress negative regulation of cell migration involved in sprouting angiogenesis positive regulation of neural precursor cell proliferation positive regulation of stem cell proliferation response to amphetamine neuron differentiation chromatin organization chromatin remodeling chromatin silencing response to activity cellular response to lipopolysaccharide regulation of histone H3-K9 acetylation
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