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Rockland
Phospho-HDAC5 (Ser661) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-HDAC5 (Ser661) Antibody (600-401-J21) in WB
Western Blot of Rabbit anti-HDAC5 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-00... View More
Product Details
600-401-J21
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding phosphoserine 661 of human HDAC5.
Conjugate
Form
Liquid
Concentration
1.12 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific towards HDAC5. A BLAST analysis was used to suggest cross-reactivity with Human based on 100% sequence homology. Cross-reactivity with HDAC5 from other sources has not been determined.
Target Information
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen NY-CO-9; DKFZp779K1053; FLJ90614; HD5; HD7; HD7b; HD9; HDAC; HDAC-5; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; Histone deacetylase 5; KIAA0744
Gene Aliases: HD5; HDAC5; KIAA0600; NY-CO-9
UniProt ID: (Human) Q9UQL6
Entrez Gene ID: (Human) 10014
RNA polymerase III transcription factor binding
core promoter binding
chromatin binding
histone deacetylase activity
protein kinase C binding
protein binding
transcription factor binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylase activity
histone deacetylase binding
transcription regulatory region DNA binding
metal ion binding
repressing transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
chromatin remodeling
chromatin silencing
transcription, DNA-templated
protein deacetylation
inflammatory response
regulation of myotube differentiation
negative regulation of myotube differentiation
response to activity
chromatin modification
histone deacetylation
neuron differentiation
B cell differentiation
cellular response to insulin stimulus
regulation of gene expression, epigenetic
B cell activation
response to cocaine
response to drug
regulation of protein binding
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
histone H3 deacetylation
cellular response to lipopolysaccharide
negative regulation of cell migration involved in sprouting angiogenesis
regulation of histone H3-K9 acetylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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