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Phospho-HMGN (Ser20, Ser24) Antibody (600-401-898) in IHC (P)
Rocklands affinity purified anti-HMGN pS20/pS24 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red sign... View More
Product Details
600-401-898
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-28 of human HMGN protein (see below).
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues (other HMGN proteins have this conserved sequence but at other residue positions). Reactivity occurs against human HMGN pS20/pS24 proteins and the antibody is specific for the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with HMGN proteins from Xenopus, chicken, mouse, bovine, dog, orangutan, rat and swine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. HMG-14 and HMG-17 also function as architectural elements, which alter the structure of the chromatin fiber and enhance transcription from chromatin templates. HMG-14/17 proteins modify the nucleosomal organization of the 30 nm chromatin fiber and mediate the unfolding of the higher order chromatin structure thereby facilitating access to the underlying DNA sequence. Clustering of architectural elements, such as HMG proteins and linker histone subtypes into distinct domains, may lead to structural and functional heterogeneity along the chromatin fiber. In addition, HMG-14 and HMG-17 have been identified as constitutive components of mouse oocyte and embryonic chromatin that establish a link between the structure of embryonic chromatin and the normal progression of embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ27265; FLJ31471; High mobility group nucleosome-binding domain-containing protein 1; high-mobility group (nonhistone chromosomal) protein 14; high-mobility group nucleosome binding 1; high-mobility group nucleosome binding domain 1; HMG 14; HMGN 1; MGC104230; MGC117425; Non-histone chromosomal protein HMG-14; nonhistone chromosomal protein HMG-14
Gene Aliases: HMG14; HMGN1
UniProt ID: (Human) P05114
Entrez Gene ID: (Human) 3150
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