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Invitrogen
Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), eFluor™ 570, eBioscience™
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FIGURE: 1 / 7
Phospho-Histone H2A.X (Ser139) Antibody (41-9865-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized MCF-7 cells treated with 10 uM camptothecin for 30 minutes (left) or immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue (right) using 1 µg/mL of Anti-Human/Mouse phospho-H2AX (S139) eFluor® 570. Nuclei are stained with DAPI. Colocalization of signal with DAPI appears pink.
Product Details
41-9865-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CR55T33
Conjugate
Excitation/Emission Max
556/569 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573654
Product Specific Information
Description: The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair.
The monoclonal antibody CR55T33 recognizes a single band of approximately 15 kDa on reduced cell lysates from Jurkat cells stimulated with etoposide.
Applications Reported: This CR55T33 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This CR55T33 antibody has been tested by immunocytochemistry of methanol-fixed cells and can be used at less than or equal to 1 µg/mL. This CR55T33 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gamma H2AX; H2A histone family, member X; H2a/x; H2AX histone; histone 5 protein 2ax; Histone H2A.X; Histone H2a/x; Histone H2AX
Gene Aliases: AW228881; gammaH2ax; H2A.X; H2A/X; H2AFX; H2AX; Hist5-2ax
UniProt ID: (Human) P16104, (Mouse) P27661
Entrez Gene ID: (Human) 3014, (Mouse) 15270
DNA damage checkpoint
double-strand break repair via homologous recombination
double-strand break repair
double-strand break repair via nonhomologous end joining
nucleosome assembly
chromatin silencing
cellular response to DNA damage stimulus
spermatogenesis
response to ionizing radiation
viral process
cerebral cortex development
positive regulation of DNA repair
meiotic cell cycle
cellular response to gamma radiation
cellular senescence
DNA repair
DNA recombination
cell cycle
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