Invitrogen

Phospho-IGF1R (Tyr1346) Polyclonal Antibody

1 Reference
View all (40) IGF1R (CD221) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-104773

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human IGF1R(Accession P08069), corresponding to amino acid residues around phosphorylated Tyr1346.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816246

Product Specific Information

Antibody detects endogenous levels of IGF1 Receptor only when phosphorylated at Tyrosine 1346.

Target Information

IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF-I receptor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2

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Gene Aliases: A330103N21Rik; CD221; D930020L01; hyft; IGF-1R; IGF1R; IGFIR; IGFIRC; IGFR; Igfr1; JTK13

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UniProt ID: (Human) P08069, (Rat) P24062, (Mouse) Q60751

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Entrez Gene ID: (Human) 3480, (Rat) 25718, (Mouse) 16001

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Function(s)
protein tyrosine kinase activity insulin-like growth factor-activated receptor activity insulin receptor binding protein binding insulin-like growth factor binding ATP binding insulin-like growth factor I binding identical protein binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding G-protein alpha-subunit binding nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
immune response signal transduction positive regulation of cell proliferation insulin receptor signaling pathway phosphatidylinositol 3-kinase signaling positive regulation of cell migration peptidyl-tyrosine autophosphorylation negative regulation of apoptotic process positive regulation of DNA replication regulation of JNK cascade protein autophosphorylation insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein tetramerization inactivation of MAPKK activity protein phosphorylation axonogenesis brain development epidermis development organ morphogenesis negative regulation of muscle cell apoptotic process establishment of cell polarity male sex determination mammary gland development exocrine pancreas development neuron projection development positive regulation of cytokinesis response to vitamin E negative regulation of MAPK cascade positive regulation of MAPK cascade negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of mitotic nuclear division positive regulation of DNA metabolic process protein heterooligomerization positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling prostate gland epithelium morphogenesis positive regulation of steroid hormone biosynthetic process transmembrane receptor protein tyrosine kinase signaling pathway phosphorylation
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