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Phospho-IKK alpha/beta (Ser176, Ser180) Antibody (710676) in ICC/IF
Time course showing induction of the TNF-a signaling cascade upon treatment: Cellular localization of proteins in the NF-KB signaling pathway was detected upon treatment of HeLa cells with TNF alpha (50 ng/mL) for 5, 10 and 25 min. Fixed and permeabilized cells were stained with Anti-IKK total Recombinant Rabbit Superclonal™ Antibody (Product # 710719, 1 µg/mL) or Anti-IKK alpha/beta (pS176/pS180) Recombina... View More
Product Details
710676
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
7HCLC
Immunogen
phosphopeptide corresponding to human IKK alpha (Ser176/180)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532752
Product Specific Information
This antibody is predicted to react with Dog, Rabbit, Pig, Mouse, Rat, Bovine and Goat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkB kinase alpha subunit; IKK-1; IKK-2; IKK-a kinase; IKK-B; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16
Gene Aliases: CHUK; IKBKA; IKBKB; IKK-alpha; IKK-beta; IKK1; IKK2; IKKA; IKKB; IMD15; NFKBIKA; NFKBIKB; TCF16
UniProt ID: (Human) O15111, (Human) O14920
Entrez Gene ID: (Human) 1147, (Human) 3551
stimulatory C-type lectin receptor signaling pathway
skeletal muscle contraction
protein phosphorylation
inflammatory response
immune response
I-kappaB kinase/NF-kappaB signaling
I-kappaB phosphorylation
Rho protein signal transduction
response to virus
response to toxic substance
anatomical structure morphogenesis
response to acetate
regulation of tumor necrosis factor-mediated signaling pathway
response to lipopolysaccharide
response to hydroperoxide
TRIF-dependent toll-like receptor signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
response to drug
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to amino acid
innate immune response
positive regulation of transcription from RNA polymerase II promoter
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
striated muscle cell differentiation
stress-activated MAPK cascade
nucleotide-binding oligomerization domain containing signaling pathway
cellular response to tumor necrosis factor
positive regulation of cell proliferation
positive regulation of neuron projection development
cortical actin cytoskeleton organization
neuron projection development
tumor necrosis factor-mediated signaling pathway
negative regulation of myosin-light-chain-phosphatase activity
regulation of phosphorylation
serine phosphorylation of STAT protein
negative regulation of apoptotic process
positive regulation of transcription, DNA-templated
interleukin-1-mediated signaling pathway
response to fatty acid
establishment of protein localization to plasma membrane
positive regulation of neuron death
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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