Invitrogen

Phospho-IKK alpha/beta (Ser180, Ser181) Polyclonal Antibody

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View all (16) IKK alpha/beta antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-104696

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CHUK(Accession O15111), corresponding to amino acid residues around phosphorylated Ser180.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816169

Product Specific Information

Antibody detects endogenous levels of IKK alpha/ beta only when phosphorylated at Serine 180/181.

Target Information

IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I kappa B kinase 1; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkappaB kinase alpha; IkB kinase alpha subunit; IKK alpha; IKK-1; IKK-2; IKK-A; IKK-a kinase; IKK-B; IKK-beta; IKK2; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16

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Gene Aliases: AI132552; AI256658; AIM-1; CHUK; Chuk1; Fbx24; Fbxo24; IKBKA; IKBKB; IKK-2; IKK-alpha; IKK-beta; IKK1; IKK2; IKK[b]; IKKA; IKKB; IKKbeta; IMD15; NFKBIKA; NFKBIKB; TCF16

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UniProt ID: (Human) O15111, (Human) O14920, (Mouse) O88351, (Rat) Q9QY78

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Entrez Gene ID: (Human) 1147, (Mouse) 12675, (Rat) 309361, (Human) 3551, (Mouse) 16150, (Rat) 84351

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Function(s)
protein kinase activity protein binding ATP binding IkappaB kinase activity protein homodimerization activity protein heterodimerization activity scaffold protein binding nucleotide binding protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding
Process(es)
B cell homeostasis protein phosphorylation I-kappaB phosphorylation positive regulation of cell proliferation positive regulation of sodium ion transport phosphorylation signal transduction by protein phosphorylation neuron projection development serine phosphorylation of STAT protein positive regulation of transcription from RNA polymerase II promoter positive regulation of NF-kappaB transcription factor activity cellular response to tumor necrosis factor positive regulation of neuron death positive regulation of cation channel activity morphogenesis of an epithelial sheet Rho protein signal transduction lactation response to organic substance osteoclast differentiation response to lipopolysaccharide mammary gland epithelial cell proliferation response to muscle stretch odontogenesis of dentin-containing tooth positive regulation of I-kappaB kinase/NF-kappaB signaling striated muscle cell differentiation mammary gland alveolus development stimulatory C-type lectin receptor signaling pathway skeletal muscle contraction inflammatory response immune response I-kappaB kinase/NF-kappaB signaling response to virus response to toxic substance anatomical structure morphogenesis response to acetate regulation of tumor necrosis factor-mediated signaling pathway response to hydroperoxide TRIF-dependent toll-like receptor signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway response to drug response to amino acid innate immune response T cell receptor signaling pathway stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway positive regulation of neuron projection development cortical actin cytoskeleton organization tumor necrosis factor-mediated signaling pathway negative regulation of myosin-light-chain-phosphatase activity regulation of phosphorylation negative regulation of apoptotic process positive regulation of transcription, DNA-templated interleukin-1-mediated signaling pathway response to fatty acid establishment of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly
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