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Phospho-INSR (Tyr1158) Antibody (710101) in ICC/IF
Immunofluorescence analysis of Phospho-IR pTyr1158 was performed using 70% confluent log phase HeLa cells treated with 10 nM Insulin for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-IR pTyr1158 Recombinant Rabbit Superclonal™ Antibody ... View More
Product Details
710101
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
8HCLC
Immunogen
Phosphopeptide corresponding to amino acids 1153-1162 of human Insulin receptor
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532564
Product Specific Information
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake. INSR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in INSR. In humans, the INSR gene is located on chromosome 19. Defects in INSR are the cause of various insulin resistance syndromes and IGF-1R defects may also cause some forms of growth retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD 220; CD220; CD220 antigen; CD220 beta; CD221; HHF 5; IGF-I receptor; Insulin receptor; IR; IR 1; IR beta; IR-1; IR1
Gene Aliases: CD220; HHF5; INSR
UniProt ID: (Human) P06213
Entrez Gene ID: (Human) 3643
protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor receptor binding
protein binding
ATP binding
GTP binding
protein phosphatase binding
lipoic acid binding
insulin-like growth factor I binding
insulin-like growth factor II binding
protein complex binding
3-phosphoinositide-dependent protein kinase binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
PTB domain binding
activation of MAPK activity
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
heart morphogenesis
carbohydrate metabolic process
regulation of transcription, DNA-templated
G-protein coupled receptor signaling pathway
positive regulation of cell proliferation
insulin receptor signaling pathway
epidermis development
male gonad development
response to manganese ion
regulation of hydrogen peroxide metabolic process
positive regulation of glycoprotein biosynthetic process
negative regulation of gene expression
response to activity
peptidyl-tyrosine phosphorylation
transformation of host cell by virus
signal transduction by protein phosphorylation
male sex determination
adrenal gland development
positive regulation of cell migration
exocrine pancreas development
activation of protein kinase activity
activation of protein kinase B activity
response to estradiol
negative regulation of transporter activity
cellular response to insulin stimulus
response to vitamin D
response to testosterone
response to tumor necrosis factor
peptidyl-tyrosine autophosphorylation
glucose homeostasis
positive regulation of MAPK cascade
positive regulation of nitric oxide biosynthetic process
fat cell differentiation
response to ethanol
positive regulation of glycogen biosynthetic process
positive regulation of DNA replication
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of embryonic development
positive regulation of glucose import
protein autophosphorylation
positive regulation of developmental growth
protein heterotetramerization
response to glucocorticoid
positive regulation of meiotic cell cycle
positive regulation of protein kinase B signaling
positive regulation of respiratory burst
cellular response to growth factor stimulus
regulation of female gonad development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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