Invitrogen

Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Polyclonal Antibody

View all (7) IR/IGF1R antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-121332

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RINSR (NP_0001992)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.42 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914904

Product Specific Information

Positive test controls include: 293T.

Immunogen sequence: YETDYY

Target Information

Insulin and Insulin-like Growth Factor receptors are transmembrane tyrosine kinase receptors that play critical roles in development, cell growth and metabolism. Insulin/Insulin-like growth factor-1 binding to the extracellular domain of IR and IGFR leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R). Autophosphorylation of the tyrosine sites within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 or 1135), followed by phosphorylation at tyrosines 1158 (IGF-1R tyrosine 1131), 1163 (IGF-1R tyrosine 1136), resulting in the full activation of IR and IGF1R.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD220; CD221; IGF-I receptor; IGF-I Receptor beta; IGF1 Receptor; IGF1 Receptor beta; IGF1R beta; Insulin receptor; insulin receptor preproprotein; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IR; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2

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Gene Aliases: 4932439J01Rik; A330103N21Rik; CD220; CD221; D630014A15Rik; D930020L01; HHF5; hyft; IGF-1R; IGF1R; IGFIR; IGFIRC; IGFR; Igfr1; INSR; IR; IR-A; IR-B; JTK13

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UniProt ID: (Human) P08069, (Human) P06213, (Rat) P24062, (Mouse) Q60751, (Mouse) P15208

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Entrez Gene ID: (Human) 3480, (Human) 3643, (Rat) 25718, (Mouse) 16001, (Rat) 24954, (Mouse) 16337

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Function(s)
protein tyrosine kinase activity insulin-like growth factor-activated receptor activity insulin receptor binding protein binding insulin-like growth factor binding ATP binding insulin-like growth factor I binding identical protein binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding PTB domain binding G-protein alpha-subunit binding nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding
Process(es)
activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis carbohydrate metabolic process regulation of transcription, DNA-templated G-protein coupled receptor signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway epidermis development male gonad development response to manganese ion regulation of hydrogen peroxide metabolic process positive regulation of glycoprotein biosynthetic process negative regulation of gene expression response to activity peptidyl-tyrosine phosphorylation transformation of host cell by virus signal transduction by protein phosphorylation male sex determination adrenal gland development positive regulation of cell migration exocrine pancreas development activation of protein kinase activity activation of protein kinase B activity response to estradiol negative regulation of transporter activity cellular response to insulin stimulus response to vitamin D response to testosterone response to tumor necrosis factor peptidyl-tyrosine autophosphorylation glucose homeostasis positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process fat cell differentiation response to ethanol positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of embryonic development positive regulation of glucose import protein autophosphorylation positive regulation of developmental growth protein heterotetramerization response to glucocorticoid positive regulation of meiotic cell cycle positive regulation of protein kinase B signaling positive regulation of respiratory burst cellular response to growth factor stimulus regulation of female gonad development immune response signal transduction phosphatidylinositol 3-kinase signaling negative regulation of apoptotic process regulation of JNK cascade insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein tetramerization inactivation of MAPKK activity protein phosphorylation axonogenesis brain development organ morphogenesis negative regulation of muscle cell apoptotic process establishment of cell polarity mammary gland development neuron projection development positive regulation of cytokinesis response to vitamin E negative regulation of MAPK cascade negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of DNA metabolic process protein heterooligomerization negative regulation of protein kinase B signaling prostate gland epithelium morphogenesis positive regulation of steroid hormone biosynthetic process transmembrane receptor protein tyrosine kinase signaling pathway response to hormone response to glucose response to nutrient levels response to insulin positive regulation of phosphorylation phosphorylation intracellular signal transduction
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