Invitrogen

Phospho-IRE1 alpha (Ser724) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-143627

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser724 of mouse IRE1, conjugated to keyhole limpet hemocyanin (KLH).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 150mM NaCl

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942856

Product Specific Information

Specific for endogenous levels of the ~130 kDa GluR1 protein phosphorylated at Ser724. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Target Information

ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Endoplasmic reticulum-to-nucleus signaling 1; ern1; FLJ30999; inositol-requiring 1 alpha; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; IRE1p antibody; MGC163277; MGC163279; Serine/threonine-protein kinase/endoribonuclease IRE1

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Gene Aliases: 9030414B18Rik; AI225830; C85377; Ern1; Ire1; Ire1a; Ire1alpha; Ire1p

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UniProt ID: (Mouse) Q9EQY0

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Entrez Gene ID: (Mouse) 78943

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Function(s)
nucleotide binding magnesium ion binding catalytic activity endonuclease activity endoribonuclease activity ribonuclease activity protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity bidentate ribonuclease III activity transferase activity transferase activity, transferring phosphorus-containing groups hydrolase activity enzyme binding Hsp70 protein binding identical protein binding protein homodimerization activity ADP binding metal ion binding Hsp90 protein binding
Process(es)
endothelial cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated mRNA cleavage mRNA processing protein phosphorylation apoptotic process response to unfolded protein activation of signaling protein activity involved in unfolded protein response cell cycle arrest activation of JUN kinase activity metabolic process phosphorylation endoplasmic reticulum unfolded protein response mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response positive regulation of RNA splicing response to endoplasmic reticulum stress cellular response to vascular endothelial growth factor stimulus peptidyl-serine autophosphorylation IRE1-mediated unfolded protein response protein autophosphorylation mRNA splicing, via endonucleolytic cleavage and ligation mRNA endonucleolytic cleavage involved in unfolded protein response intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to glucose stimulus RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of endoplasmic reticulum unfolded protein response insulin metabolic process peptidyl-serine trans-autophosphorylation
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