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Invitrogen
Phospho-IkappaB beta (Ser23) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-IkappaB beta (Ser23) Antibody (PA5-104999) in ICC/IF
Immunocytochemistry analysis of Phospho-IkappaB beta (Ser23) in HepG2 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-IkappaB beta (Ser23) polyclonal antibody (Product # PA5-104999) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 c... View More
Product Details
PA5-104999
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NFKBIB(Accession Q15653), corresponding to amino acid residues around phosphorylated Ser23.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816472
Product Specific Information
Antibody detects endogenous levels of IKB beta only when phosphorylated at Serine 23.
Target Information
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB. The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKK alpha and IKK beta serve as the catalytic subunits of the kinase and IKK gamma serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes, resulting in kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I kappa B beta; I-kappa-B-beta; ikappaBbeta; IKB B; IKB beta; IkB-B; ikB-beta; IkBbeta; NF kappa BIB; NF-kappa-B inhibitor beta; NF-kappa-BIB; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta; Thyroid receptor; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP 9; TRIP-9
Gene Aliases: I(Kappa)B(beta); I-kappa-B-beta; IKappaBbeta; IkB; ikB-B; IKB-beta; IKBB; NF-kappa-BIB; NFKBIB; TRIP9
UniProt ID: (Human) Q15653, (Mouse) Q60778
Entrez Gene ID: (Human) 4793, (Rat) 81525, (Mouse) 18036
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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